REGULATION OF A YEAST HSP70 GENE BY A CAMP RESPONSIVE TRANSCRIPTIONAL CONTROL ELEMENT
REGULATION OF A YEAST HSP70 GENE BY A CAMP RESPONSIVE TRANSCRIPTIONAL CONTROL ELEMENT
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DOI：
10.1002/j.1460-2075.1990.tb07435.x
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发表时间：
1990-08-01
期刊：
影响因子：
11.4
通讯作者：
CRAIG, EA
中科院分区：
文献类型：
--
作者：
BOORSTEIN, WR;CRAIG, EA
HSP70 基因在应对应激以及各种细胞和发育事件时表现出复杂的调节。酿酒酵母的 SSA3 HSP70 基因在营养限制条件下在转录水平被激活。对缺失的分析表明，存在于先前鉴定的热休克元件（UASHS）上游和下游的顺式作用DNA序列介导了这种调节。 SSA3 的 35 bp 区域与 UASHS 不同，包含能够在酵母生命周期的双轴转变和稳定期激活异源启动子的序列；该区域被指定为上游激活序列，UASPDS。 UASPDS 驱动的表达受 RAS/cAMP 通路调节。 cAMP 依赖性蛋白激酶活性降低导致 SSAB 启动子的 UASPDS 依赖性激活，而组成型 cAMP 依赖性蛋白激酶活性则阻止 UASPDS 介导的转录，即使在通常会导致完全激活的生长条件下也是如此。尽管在 UASPDS 促进转录的条件下，单独的热休克元件不表现出 UAS 活性，但 UASHS 与 UASPDS 积极相互作用，介导高水平的 SSA3 转录，以响应营养限制和降低的细胞内 cAMP 浓度。这种相互作用与两个元件的精确间距和相对方向无关。
HSP70 genes exhibit complex regulation in response to stress and a variety of cellular and developmental events. The SSA3 HSP70 gene of Saccharomyces cerevisiae is activated at the transcriptional level which conditions of nutrient limitation. Analysis of deletions revealed that cis-acting DNA sequences present immediatedly upstream and downstream of the previously identified heat shock elements (UASHS) mediate this regulation. A 35 bp region of SSA3, distinct from UASHS, contains sequences capable of activating a heterologous promoter following the diauxic shift and in the stationary phase of the yeast life cycle; this region has been designated an upstream activating sequence, UASPDS. Expression driven by UASPDS is regulated by the RAS/cAMP pathway. Reduced cAMP dependent protein kinase activity results in UASPDS dependent activation of the SSAB promoter while constitutive cAMP dependent protein kinase activity prevents UASPDS mediated transcription, even under growth conditions that would normally result in full activation. Although the heat shock element alone exhibits no UAS activity under conditions in which UASPDS promotes transcription, UASHS interacts positively with UASPDS to mediate high levels of SSA3 transcription in response to nutrient limitation and lowered intracellular cAMP concentration. This interaction is independent of the precise spacing and relative orientation of the two elements.