Partition of parinaroylphosphatidylethanolamines and parinaroylphosphatidylglycerols in immiscible phospholipid mixtures.
Partition of parinaroylphosphatidylethanolamines and parinaroylphosphatidylglycerols in immiscible phospholipid mixtures.
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仲芳酰磷脂酰乙醇胺和仲芳酰磷脂酰甘油在不混溶的磷脂混合物中的分配。
DOI：
10.1016/0005-2736(90)90130-g
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Welti,R
中科院分区：
文献类型：
--
作者：
Martin,LR;Avery,RB;Welti,R
研究了两种parinaroyl磷脂酰乙醇胺和两种parinaroyl磷脂酰甘油在固相和液相磷脂之间的分配。荧光量子产率和荧光偏振测量用于计算Kps/t，即每种探针的固体与流体的分配系数（Sklar，L.A.，Miljanich，G. P.和Dratz，E. A.（1979）Biochemistry 18，1707-1716）。在不混溶的二棕榈酰磷脂酰胆碱和二亚油酰磷脂酰胆碱混合物中，1-棕榈酰-2-反式-parinaroylphosphatidylethanolamine和1-棕榈酰-2-反式-parinaroylphosphatidylglycerol均优先分配到固相脂质中，平均Kps/t值（由量子产率计算）分别为3.4 ± 1.5和2.1 ± 0.7。相比之下，1-油酰基-2-cis-parinaroylphosphatidylethanolamine和1-油酰基-2-cis-parinaroylphosphatidylglycerol在相同的模型系统中优先分配到流体相脂质中，平均Kps/t值（由量子产率计算）分别为0.44 ± 0.26和0.16 ± 0.07。相同的四parinaroyl磷脂在固相二肉豆蔻酰磷脂酰乙醇胺和流体相二亚油酰磷脂酰甘油的混合物中的荧光偏振数据是类似的那些在不混溶的磷脂酰胆碱系统中获得的，表明这些探针的分区是不是强烈依赖于头基。这些荧光探针的分配特性的知识是有关使用这些探针在调查ofEscherichia coli内膜脂质的相行为，因为磷脂酰乙醇胺和磷脂酰甘油物种约占这些脂质的95%。
Partitioning of two parinaroyl phosphatidylethanolamines and two parinaroyl phosphatidylglycerols between solid and fluid phase phospholipids was examined. Fluorescence quantum yields and fluorescence polarization measurements were used to calculateKps/t, the solid to fluid partition coefficient of each probe (Sklar, L.A., Miljanich, G.P. and Dratz, E.A. (1979) Biochemistry 18, 1707–1716). In the immiscible mixture dipalmitoylphosphatidylcholine and dilinoleylphosphatidylcholine, both 1-palmitoyl-2-trans-parinaroylphosphatidylethanolamine and 1-palmitoyl-2-trans-parinaroylphosphatidylglycerol partitioned preferentially into solid phase lipid with meanKps/tvalues (calculated from quantum yields) of 3.4 ± 1.5 and 2.1 ± 0.7, respectively. In contrast, 1-oleoyl-2-cis-parinaroylphosphatidylethanolamine and 1-oleoyl-2-cis-parinaroylphosphatidylglycerol partitioned preferentially into fluid phase lipid in the same model system with meanKps/tvalues (calculated from quantum yields) of 0.44 ± 0.26 and 0.16 ± 0.07, respectively. Fluorescence polarization data on the same four parinaroyl phospholipids in mixtures of solid-phase dimyristoylphosphatidyl ethanolamine and fluid-phase dilinoleoylphosphatidylglycerol were similar to those obtained in the immiscible phosphatidylcholine system, demonstrating that the partitioning of these probes is not strongly dependent on head group. Knowledge of the partition properties of these fluorescent probes is relevant to use of these probes in investigation of the phase behavior ofEscherichia coliinner membrane lipids, since phosphatidylethanolamine and phosphatidylglycerol species account for approximately 95% of these lipids.