EFFECTS OF INTRAVENTRICULAR-INJECTIONS OF 6-HYDROXYDOPAMINE ON ANTERIOR-PITUITARY CELL-PROLIFERATION
EFFECTS OF INTRAVENTRICULAR-INJECTIONS OF 6-HYDROXYDOPAMINE ON ANTERIOR-PITUITARY CELL-PROLIFERATION
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DOI：
10.1002/ar.1092080312
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发表时间：
1984-01-01
影响因子：
--
通讯作者：
REITER, RJ
中科院分区：
文献类型：
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作者：
LEWINSKI, A;KONOPACKI, J;REITER, RJ
采用秋水仙碱中期停滞技术，观察脑室注射6-羟基多巴胺(6-OHDA；200微克/20微升载体)对大鼠垂体前叶细胞增殖的影响。在注射6-OHDA或6-OHDA+去甲基米帕明(DMI，25 mg/kg体重，ip)的动物中，平均有丝分裂活动率(MMAR)在48h开始较低，在注射药物后96h和144h观察到MMAR的增加。给药后144h，6-OHDA组和6-OHDA+DMI组的MMAR值均显著高于对照组。这种MMAR的增加是由于嗜酸性和嫌色细胞的增殖增强所致。注射6-OHDA后48h MMAR降低可能是由于受损神经末梢释放多巴胺所致，给药后96和144h MMAR增强(以6-OHDA+DMI组最为明显)，可能与多巴胺缺乏对垂体前叶细胞增殖的调控有关。
Effects of intraventricular injections of 6-hydroxydopamine (6-OHDA; 200 .mu.g/20 .mu.l vehicle) on anterior pituitary cell proliferation in rats was investigated by means of the colchicine metaphase-arrest technique. In those groups of animals receiving 6-OHDA alone or 6-OHDA plus desmethylimipramine (DMI, 25 mg/kg body weight i.p.), where the mean mitotic activity rates (MMAR) were initially low at 48 h, an increase of MMAR was observed at 96 and 144 h after the drug injections. At 144 h after drug adminstration, the MMAR values in the 6-OHDA-injected group and in the 6-OHDA + DMI-treated group were significantly higher than those in the control groups. This increase of MMAR resulted from the enhancement of acidophilic and chromophobe cell proliferation. The low MMAR at 48 h after 6-OHDA injection are probably a result of dopamine release from damaged nerve endings; the enhancement of MMAR (particularly evident in the 6-OHDA + DMI-treated group) at 96 and 144 h after drug administration is presumably related to a deficiency of dopaminergic control of anterior pituitary cell proliferation.