Human oral cavity as a model for the study of genome-genome interactions
Human oral cavity as a model for the study of genome-genome interactions
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DOI：
10.2307/1543559
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发表时间：
2003-04-01
影响因子：
1.6
通讯作者：
Kolenbrander, PE
中科院分区：
文献类型：
--
作者：
Foster, JS;Palmer, RJ;Kolenbrander, PE
口腔微生物群落中可培养细菌的巨大多样性加上实验的可及性使人类口腔成为研究基因组间相互作用的有价值的模型。口腔细菌复杂的相互作用导致口腔表面生物膜的形成。一种被认为对生物膜形成很重要的机制是细菌伙伴的共聚集。在本文中，我们研究了口腔生物膜中共聚集的作用，并制定了阐明口腔生物膜内保留的细菌物种的空间组织的方案。为了探索这些问题，我们采用了两个实验系统：唾液涂层流通池和可回收釉质芯片。通过流动池研究，我们确定共聚集可以极大地影响口腔细菌物种生长和保留在正在发育的生物膜内的能力。为了检查口腔生物膜的空间结构，开发了荧光原位杂交方案，成功针对口腔微生物群落的特定成员。总之，这些方法提供了对口腔生物膜发展的深入了解，并扩大了我们对基因组间相互作用的理解。
The enormous diversity of culturable bacteria within the oral microbial community coupled with experimental accessibility renders the human oral cavity a valuable model to investigate genome-genome interactions. The complex interactions of oral bacteria result in the formation of biofilms on the surfaces of the oral cavity. One mechanism thought to be important in biofilm formation is the coaggregation of bacterial partners. In this paper, we examine the role of coaggregation in oral biofilms and develop protocols to elucidate the spatial organization of bacterial species retained within oral biofilms. To explore these issues, we have employed two experimental systems: the saliva-coated flowcell and the retrievable enamel chip. From flowcell studies, we have determined that coaggregation can greatly influence the ability of an oral bacterial species to grow and be retained within the developing biofilm. To examine the spatial architecture of oral biofilms, fluorescent in situ hybridization protocols were developed that successfully target specific members of the oral microbial community. Together, these approaches provide insight into the development of oral biofilms and expand our understanding of genome-genome interactions.