Stress signaling in response to polycyclic aromatic hydrocarbon exposure in Arabidopsis thaliana involves a nucleoside diphosphate kinase, NDPK-3
Stress signaling in response to polycyclic aromatic hydrocarbon exposure in Arabidopsis thaliana involves a nucleoside diphosphate kinase, NDPK-3
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DOI：
10.1007/s00425-014-2161-8
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发表时间：
2015-01-01
期刊：
影响因子：
4.3
通讯作者：
Colon-Carmona, Adan
中科院分区：
文献类型：
--
作者：
Liu, Hong;Weisman, David;Colon-Carmona, Adan
该研究首次揭示了植物对单一PAH胁迫的蛋白质组反应，并表明NDPK3是拟南芥对菲胁迫反应的正调节因子。多环芳烃（PAH）是一种高度致癌的污染物，是碳基燃料燃烧的副产品，并且往往会在环境中长期存在。 PAH 在植物中引起复杂的破坏性反应，之前在生理、生化和转录水平上的研究表明，活性氧 (ROS) 和氧化应激在 PAH 反应中发挥着重要作用。然而，蛋白质组学反应在很大程度上仍未得到探索。这项研究假设拟南芥对菲（一种多环芳烃模型）的蛋白质组反应将包括强烈的氧化应激特征，并将提供相关潜在信号分子的线索。为了探索蛋白质组特征，我们进行了 2D-PAGE 实验，并鉴定了 30 种蛋白质水平发生显着改变，包括过氧化氢酶 (CAT)、抗坏血酸过氧化物酶 (APX)、过氧化还原酶 (POD)、谷胱甘肽-S-转移酶和谷胱甘肽还原酶。核苷二磷酸激酶 3 (NDPK-3) 的表达也上调，这是一种已知具有代谢和应激信号传导功能的蛋白质。为了确定 NDPK-3 是否在氧化应激反应上游发挥作用，我们测量了 NDPK-3 过表达、功能缺失敲除和野生型植物品系中应激反应酶的水平。在NDPK-3过表达子中，APX、CAT、POD以及超氧化物歧化酶的酶活性均较野生型有所增加；在 NDPK-3 敲除系中，这些酶的活性降低。这种模式发生在未经处理和菲处理的植物中。这些数据支持一个模型，其中 NDPK-3 是拟南芥对 PAH 胁迫反应的正调节因子。
The study is the first to reveal the proteomic response in plants to a single PAH stress, and indicates that NDPK3 is a positive regulator in the Arabidopsis response to phenanthrene stress.Polycyclic aromatic hydrocarbons (PAHs) are highly carcinogenic pollutants that are byproducts of carbon-based fuel combustion, and tend to persist in the environment for long periods of time. PAHs elicit complex, damaging responses in plants, and prior research at the physiological, biochemical, and transcriptional levels has indicated that reactive oxygen species (ROS) and oxidative stress play major roles in the PAH response. However, the proteomic response has remained largely unexplored. This study hypothesized that the proteomic response in Arabidopsis thaliana to phenanthrene, a model PAH, would include a strong oxidative stress signature, and would provide leads to potential signaling molecules involved. To explore that proteomic signature, we performed 2D-PAGE experiments and identified 30 proteins levels that were significantly altered including catalases (CAT), ascorbate peroxidase (APX), peroxiredoxins (POD), glutathione-S-transferase, and glutathione reductase. Also upregulated was nucleoside diphosphate kinase 3 (NDPK-3), a protein known to have metabolic and stress signaling functions. To address whether NDPK-3 functions upstream of the oxidative stress response, we measured levels of stress-responsive enzymes in NDPK-3 overexpressor, loss-of-function knockout, and wild-type plant lines. In the NDPK-3 overexpressor, the enzyme activities of APX, CAT, POD, as well as superoxide dismutase were all increased compared to wild type; in the NDPK-3 knockout line, these enzymes had reduced activity. This pattern occurred in untreated as well as phenanthrene-treated plants. These data support a model in which NDPK-3 is a positive regulator of the Arabidopsis stress response to PAHs.