The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour
The relative influence of the topography and chemistry of TiAl6V4 surfaces on osteoblastic cell behaviour
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DOI：
10.1016/s0142-9612(00)00042-9
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发表时间：
2000-08-01
期刊：
影响因子：
14
通讯作者：
Leroy, JM
中科院分区：
文献类型：
--
作者：
Anselme, K;Linez, P;Leroy, JM
采用不同表面粗糙度的Ti6 Al 4V钛合金试样，研究了小鼠成骨细胞（MC 3 T3-E1）和原代人成骨细胞在其表面的增殖和粘附。制备机械或手动抛光的表面以分别产生非定向或定向的残余抛光槽。使用500 μ m或3 mm氧化铝颗粒制备喷砂表面。表面粗糙度参数表现出负相关性相比，增殖和粘附参数。X射线微探针化学表面微区分析表明，喷砂处理后的Ti6 Al 4V合金的表面元素组成的完全扰动。在样品表面观察到AlOx富集层。这可能导致怀疑表面粗糙度幅度和AlOx表面浓度的伴随效应对成骨细胞增殖和粘附有影响。这些发现表明，在任何生物学使用之前，对钛基植入物进行任何表面处理后进行化学表面分析具有重要意义。(C)2000爱思唯尔科技有限公司版权所有。
Proliferation and adhesion of mouse (MC3T3-E1) osteoblastic cells and primary human osteoblastic cells were carried out on Ti6Al4V titanium alloy samples with varied surface roughnesses. Mechanically or manually polished surfaces were prepared to produce respectively non-oriented or oriented residual polishing grooves. Sand-blasted surfaces were prepared using 500 mu m or 3 mm alumina particles. Surface roughness parameters showed a negative correlation in comparison to proliferation and adhesion parameters. X-ray microprobe chemical surface microanalysis showed complete disturbance of the surface element composition of the Ti6Al4V alloy following sand-blasting treatment. An AlOx-enriched layer was observed on sample surfaces. This may lead to the suspicion that the concomittant effect of surface roughness amplitude and AlOx surface concentration has an effect on osteoblastic cell proliferation and adhesion. These findings show the significance of chemical surface analysis after any surface treatment of titanium-based implants before any biological use. (C) 2000 Elsevier Science Ltd. All rights reserved.