Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodies.
Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodies.
复制标题
使用单特异性抗体鉴定 1 型和 2 型光捕获叶绿素 a/b 结合蛋白。
DOI：
10.1016/s0005-2728(05)80336-6
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Staehelin,LA
中科院分区：
文献类型：
--
作者：
Sigrist,M;Staehelin,LA
通过对不同植物光系统II （LHC II）相关光收集复合体的40多个载脂蛋白的相应基因测序，推导出了它们的氨基酸序列。这些高度保守的序列分为两大类，1型和2型，它们主要在靠近n端的少数结构域上有所不同。我们利用来自矮牵牛基因的序列，制备了针对1型和2型LHC II载脂蛋白n端最独特序列域的合成肽的多克隆单特异性抗体。在Western blots上，我们的抗1型和2型抗体与矮牵牛、番茄、菠菜和其他几种植物的捕光蛋白发生交叉反应。通过使用一种新的基于氨基（2-氨基-2-甲基-1,3-丙二醇）的凝胶体系，我们能够分解多达8种LHC II载脂蛋白。在牵牛花、番茄和菠菜的印迹上，抗1型抗体与两个或多个高分子量的LHC II多肽结合，而抗2型抗体只特异性地识别一个或两个低分子量的LHC-蛋白。在所研究的所有植物中，1型LHC II载脂蛋白比2型载脂蛋白数量更多，大小范围更大。这与迄今为止发现的较少数量的2型LHC IICABgenes是一致的。
The amino acid sequences of more than 40 apoproteins of the light-harvesting complex associated with Photosystem II (LHC II) of various plants have been deduced by sequencing their corresponding genes. These highly conserved sequences fall into two major categories, type 1 and type 2, that differ mainly in a small number of domains close to the N-terminus. We have made polyclonal, monospecific antibodies against synthetic peptides corresponding to the most unique sequence domains of the N-terminal regions of type 1 and type 2 LHC II apoproteins, using sequences derived from petunia genes. On Western blots our anti-type 1 and 2 antibodies crossreact with light-harvesting proteins of petunia, tomato, spinach and several other plants. By using an new gel-system based on ammediol (2-amino-2-methyl-1,3-propanediol), we are able to resolve up to eight LHC II apoproteins. On petunia, tomato and spinach blots the anti type 1 antibodies bind to two or more of the higher molecular weight LHC II polypeptides, whereas the anti type 2 antibodies recognize very specifically only one or two of the lower molecular weight LHC-proteins. In all plants studied, the type 1 LHC II apoproteins are more numerous and span a greater size range than the type 2 apoproteins. This is consistent with the smaller number of type 2 LHC IICABgenes that have been discovered to date.