Perinatal responsiveness to alcohol's chemosensory cues as a function of prenatal alcohol administration during gestational days 17-20 in the rat
Perinatal responsiveness to alcohol's chemosensory cues as a function of prenatal alcohol administration during gestational days 17-20 in the rat
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DOI：
10.1006/nlme.1996.0012
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发表时间：
1996-03-01
影响因子：
2.7
通讯作者：
Molina, JC
中科院分区：
文献类型：
--
作者：
Dominguez, HD;Lopez, MF;Molina, JC
当药物直接进入羊水时，临近出生的大鼠胎儿会产生酒精来源的提示。先前的证据表明，化学感觉在妊娠第17天（GD17）被检测到。在本研究中，wistar衍生的孕妇在妊娠期17-20天内接受0、1或2 g/kg/天的酒精（胃内插管）。产前治疗未能影响母胎和围产儿的不同身体参数，如胎盘重量、脐带长度、后代体重、嗅球、大脑半球和小脑的重量和/或大小。在围产期运动活动测试中评估的酒精化学感觉反应表明，当实验室内存在酒精气味时，用1和2 g/kg酒精剂量预处理的幼犬的活动率显着降低。在女性接受1或2 g/kg酒精剂量的最后一次插管程序（GD20） 1小时后，还通过头空色谱法记录了母体和胎儿血液以及羊水中的酒精浓度。记录了不同评估地点的剂量依赖性酒精浓度。正如以前的研究所表明的那样，即使在1 g/kg酒精剂量下羊水中的酒精水平也高于允许胎儿用酒精来源的线索进行化学感觉刺激的阈值。研究结果表明，母亲在怀孕的最后几天酒精中毒会导致胎儿接触到酒精的感官属性，这种经历随后会改变对这些线索的反应。(C) 1996学术出版社，Inc.
Rat fetuses proximal to birth process alcohol-derived cues when the drug is directly delivered into the amniotic fluid. Prior evidence indicates that chemosensory sensation is detected during gestational Day 17 (GD17). In the present study Wistar-derived pregnant females received 0, 1, or 2 g/kg/day of alcohol (intragastric intubation) during GDs 17-20. Prenatal treatment failed to affect different maternal-fetal and perinatal physical parameters, e.g., placenta weight, umbilical cord length, offspring's body weights, weights and/or size of the olfactory bulbs, cerebral hemispheres, and cerebellum. Alcohol chemosensory responsiveness assessed in a perinatal motor activity test, indicated that pups pretreated with the 1 and 2 g/kg alcohol dose exhibit significant decrements in their activity rate when alcohol odor is presented in the test chamber. Alcohol concentrations in maternal and fetal blood and in the amniotic fluid were also recorded through head-space chromatography 1 h after females received the last intubation procedure (GD20) with the 1 or 2 g/kg alcohol doses. Dose-dependent alcohol concentrations across the different sites of assessment were recorded. As indicated by previous studies, even the alcohol level in the amniotic fluid attained with the 1 g/kg alcohol dose is above threshold values in terms of allowing fetal chemosensory stimulation with alcohol-derived cues. The results suggest that maternal ethanol intoxication during the last days of pregnancy leads to fetal exposure to alcohol's sensory attributes and that this experience subsequently modifies responsiveness to these cues. (C) 1996 Academic Press, Inc.