STRUCTURE OF THE RATS BEHAVIOR IN THE HOT PLATE TEST
STRUCTURE OF THE RATS BEHAVIOR IN THE HOT PLATE TEST
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DOI：
10.1016/0166-4328(93)90035-o
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发表时间：
1993-09-30
影响因子：
2.7
通讯作者：
MIR, D
中科院分区：
文献类型：
--
作者：
ESPEJO, EF;MIR, D
本研究的目的是：（i）详细说明大鼠在热板试验期间的行为，（ii）通过使用行为学技术分析大鼠对伤害性热刺激的反应，以及（iii）辨别合适的模式或模式以评估大鼠对伤害性刺激的反应。采用雄性Wistar大鼠（n = 30），对每只大鼠进行一次热板试验。将平台（Socrel DS 37）保持在55.0 +/-0.5 ℃，并且暴露时间为45秒。行为被录像，并通过行为图和软件包进行分析。量化每个模式参数（频率、持续时间和首次出现的潜伏期）。聚类分析和马尔可夫分析，从而得到的树状图和马尔可夫链，分别。行为图由12种模式组成：不动嗅、行走嗅、直立、舔前爪、舔后爪、洗脸、清洁身体、跺脚、后腿缩回、倾斜姿势、跳跃和冻结。聚类分析结果表明，最常见的模式可以分为三类：嗅反应（不动嗅，走嗅），主要的毒性诱发模式（前蹄舔，后蹄舔，跺脚），逃避毒性诱发反应（倾斜姿势，跳跃）。后足退缩是一种独立的毒性诱发模式，而冻结是一种警觉行为。行为学信息可能有助于更好地阐明热板试验期间药物治疗对大鼠行为或其他现象的影响。
The objectives of the study were: (i) to elaborate an ethogram of the rat's behaviour during the hot plate test, (ii) to analyse the rat's responses to the nociceptive heat stimulus by using ethological techniques, and (iii) to discern the suitable pattern or patterns to evaluate the rat's reaction to the noxious stimulus. Male Wistar rats (n = 30) were employed, and one hot plate test was carried out for each rat. The platform (Socrel DS37) was maintained at 55.0 +/- 0.5-degrees-C, and the exposure time was 45 s. Behaviour was videotaped and analysed by an ethogram and a software package. Every pattern parameter (frequency, duration and latency for the first occurrence) was quantified. Cluster and Markovian analyses were also employed, whereby a dendrogram and a Markov chain were obtained, respectively. The ethogram was composed of twelve patterns: immobile-sniff, walk-sniff, rearing, forepaw-licking, hindpaw-licking, face-washing, body-cleaning, stamping, hindleg-withdrawal, leaning posture, jumping off, and freezing. Cluster analysis indicated that the most frequent patterns could be classified into three categories: sniffing responses (immobile-sniff, walk-sniff), primary noxious-evoked patterns (forepaw-licking, hindpaw-licking, stamping), and escape noxious-evoked responses (leaning posture, jumping off). Hindleg-withdrawal would be an independent noxious-evoked pattern, and freezing an alerting behaviour. The ethological information could be useful to better elucidate effects on rats' behaviour of drug treatment or other phenomena during the hot plate test.