CD28 is required for germinal center formation.
CD28 is required for germinal center formation.
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DOI：
10.4049/jimmunol.156.12.4576
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发表时间：
1996-06
影响因子：
4.4
通讯作者：
S. Ferguson;Shuhua Han;G. Kelsoe;C. Thompson
中科院分区：
文献类型：
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作者：
S. Ferguson;Shuhua Han;G. Kelsoe;C. Thompson
先前的研究表明，T细胞共刺激分子CD28在体液免疫的发展中起重要作用。cd28缺陷小鼠在同型转换中表现出缺陷，并且更容易受到依赖于有效Ab反应的病原体的影响。为了确定这些缺陷的基础，我们在微环境水平上检测了cd28缺陷小鼠的B细胞反应。在正常T依赖性免疫应答的早期，少量B细胞在次级淋巴组织的富T细胞区被激活，然后迁移到B细胞区。这些迁移的B细胞通过增殖扩张发现正在发育的生发中心，在这一过程中，单个细胞在重组的Ig基因中获得突变。对Ag具有较高亲和力的B细胞突变体在生发中心进行正向选择，导致记忆B细胞区室的建立。在目前的研究中，我们证明了尽管淋巴滤泡内潜在的ag反应细胞在抗原攻击后积累，但这些细胞在cd28缺陷动物中不能进行增殖扩增形成生发中心，也不会获得体细胞突变。因此，CD28激活途径是Ab对t依赖性Ags反应所必需的。隔室。在目前的研究中，我们证明了尽管淋巴滤泡内潜在的ag反应细胞在抗原攻击后积累，但这些细胞在cd28缺陷动物中不能进行增殖扩增形成生发中心，也不会获得体细胞突变。因此，CD28激活途径是Ab对t依赖性Ags反应所必需的。
Previous studies have demonstrated that the T cell costimulatory molecule, CD28, is important in the development of humoral immunity. CD28-deficient mice exhibit defects in isotype switching and are more susceptible to pathogens that depend on an effective Ab response. To determine the basis of these defects, we have examined B cell responses of CD28-deficient mice at the microenvironmental level. Early in a normal T-dependent immune response, small numbers of B cells undergo activation in the T cell-rich zone of secondary lymphoid tissues and then migrate to B cell areas. These migrant B cells found developing germinal centers by proliferative expansion, during which individual cells acquire mutations in their rearranged Ig genes. B cell mutants retaining higher affinities for Ag undergo positive selection in germinal centers, resulting in the establishment of the memory B cell compartment. In the present study, we demonstrate that although potentially Ag-reactive cells within the lymphoid follicle accumulate following antigenic challenge, these cells fail to undergo proliferative expansion to form germinal centers and do not acquire somatic mutations in CD28-deficient animals. Thus, the CD28 activation pathway is required for Ab responses to T-dependent Ags. cell compartment. In the present study, we demonstrate that although potentially Ag-reactive cells within the lymphoid follicle accumulate following antigenic challenge, these cells fail to undergo proliferative expansion to form germinal centers and do not acquire somatic mutations in CD28-deficient animals. Thus, the CD28 activation pathway is required for Ab responses to T-dependent Ags.