Arabidopsis thaliana subcellular responses to compatible Erysiphe cichoracearum infections
Arabidopsis thaliana subcellular responses to compatible Erysiphe cichoracearum infections
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DOI：
10.1111/j.1365-313x.2005.02545.x
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发表时间：
2005-11-01
期刊：
影响因子：
7.2
通讯作者：
Somerville, S
中科院分区：
文献类型：
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作者：
Koh, S;André, A;Somerville, S
通过激光扫描共聚焦显微镜观察了携带各种绿色荧光蛋白（GFP）标记细胞器的拟南芥植物的活组织中白粉病菌感染表皮细胞的亚细胞事件。在感染序列的早期，细胞质和细胞器向穿透部位移动，并在穿透钉附近积累。过氧化物酶体似乎积累优先相对于细胞质的渗透网站。吸器形成之前的另一个早期事件是一些GFP标记的质膜标记蛋白聚集成围绕渗透位点的环，其延伸穿过细胞壁边界进入相邻细胞。这一特征局限于乳头沉积的部位。吸器外膜（EHM）包裹真菌的进食结构吸器，将其与宿主细胞质分开。8个质膜标记被排除在外的EHM，并保持在一个衣领状的形成吸器颈部周围。这些观察结果支持EHM是一种独特的，专门的膜，是从质膜不同的建议。我们的研究结果表明，EHM的起源有两种可能性：质膜的内陷与膜分化;或从头合成的EHM有针对性的囊泡贩运。
Subcellular events of Erysiphe cichoracearum infections of epidermal cells were visualized in living tissues of Arabidopsis plants carrying various green fluorescent protein (GFP)-tagged organelles via laser scanning confocal microscopy. Early in the infection sequence, cytoplasm and organelles moved towards penetration sites and accumulated near penetration pegs. Peroxisomes appeared to accumulate preferentially relative to the cytoplasm at penetration sites. Another early event, which preceded haustorium formation, was the aggregation of some GFP-tagged plasma membrane marker proteins into rings around penetration sites, which extended across cell-wall boundaries into neighboring cells. This feature localized to sites where papillae were deposited. The extrahaustorial membrane (EHM) encases the fungal feeding structure, the haustorium, separating it from the host cytoplasm. Eight plasma membrane markers were excluded from the EHM and remained in a collar-like formation around the haustorial neck. These observations support the suggestions that the EHM is a unique, specialized membrane and is different from the plasma membrane. Our results suggested two possibilities for the origin of the EHM: invagination of the plasma membrane coupled with membrane differentiation; or de novo synthesis of the EHM by targeted vesicle trafficking.