Photothermal ablation of pancreatic cancer cells with hybrid iron-oxide core gold-shell nanoparticles.
Photothermal ablation of pancreatic cancer cells with hybrid iron-oxide core gold-shell nanoparticles.
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DOI：
10.2147/ijn.s47585
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发表时间：
2013
影响因子：
8
通讯作者：
Larson AC
中科院分区：
文献类型：
--
作者：
Guo Y;Zhang Z;Kim DH;Li W;Nicolai J;Procissi D;Huan Y;Han G;Omary RA;Larson AC
光热消融是一种微创方法，其通常涉及将光热敏化剂递送至靶组织。我们研究的目的是证明金纳米颗粒被胰腺癌细胞吞噬，从而允许敏化剂递送和光热消融的磁共振成像（MRI）。使用氧化铁核/金壳纳米颗粒（GoldMag®，30 nm直径;西安GoldMag生物技术有限公司，西安，中华人民共和国）。在96孔板中，放置3 × 104个PANC-1（人胰腺癌细胞系）细胞。将GoldMag（0、25或50 μg/mL）加入每个孔中，并允许细胞摄取24小时。然后将样本分为两组：一组用光热消融（7.9 W/cm 2）处理5分钟，另一组不处理。使用激光系统（BWF 5; B&W Tek，Inc，纽瓦克，DE，美国）进行光热烧蚀。使用光纤温度探头（FTP-LN 2; Photon Control Inc，Burnaby，BC，Canada）测量术中温度变化。24小时后，使用台盼蓝染色计数剩余的活细胞数;基于处理后与对照相比的活细胞总数计算细胞增殖百分比。使用7.0 T ClinScan系统（Bruker BioSpin，Ettlingen，德国）进行GoldMag摄取的MRI。温度曲线表明，随着GoldMag吸收的增加，激光照射在相应样品中产生更高的温度升高;对于0、25和50 μg/mL GoldMag，温度升高达到12.89°C、35.16°C和79.51°C。在没有光热消融的情况下，25和50 μg/mL GoldMag处理的细胞增殖百分比从100%变为71.3%和47.0%。PANC-1细胞的光热消融证明了有效的治疗反应，特别是用0、25和50 μg/mL GoldMag处理的细胞的细胞增殖降低至仅61%、21.9%和2.3%。MRI能够可视化PANC-1细胞内的GoldMag摄取。我们的研究结果表明，光热消融可能是有效的治疗胰腺癌。金磁纳米颗粒可以作为光热敏化剂，MRI是可行的量化交付。
Photothermal ablation is a minimally invasive approach, which typically involves delivery of photothermal sensitizers to targeted tissues. The purpose of our study was to demonstrate that gold nanoparticles are phagocytosed by pancreatic cancer cells, thus permitting magnetic resonance imaging (MRI) of sensitizer delivery and photothermal ablation. Iron-oxide core/gold-shell nanoparticles (GoldMag®, 30 nm diameter; Xi’an GoldMag Biotechnology Co, Xi’an, People’s Republic of China) were used. In a 96-well plate, 3 × 104 PANC-1 (human pancreatic cancer cell line) cells were placed. GoldMag (0, 25, or 50 μg/mL) was added to each well and 24 hours allowed for cellular uptake. Samples were then divided into two groups: one treated with photothermal ablation (7.9 W/cm2) for 5 minutes, the other not treated. Photothermal ablation was performed using laser system (BWF5; B&W Tek, Inc, Newark, DE, USA). Intraprocedural temperature changes were measured using a fiber optic temperature probe (FTP-LN2; Photon Control Inc, Burnaby, BC, Canada). After 24 hours, the remaining number of viable cells was counted using trypan blue staining; cell proliferation percentage was calculated based on the total number of viable cells after treatment compared with control. MRI of GoldMag uptake was performed using a 7.0T ClinScan system (Bruker BioSpin, Ettlingen, Germany). Temperature curves demonstrated that with increased GoldMag uptake, laser irradiation produced higher temperature elevations in the corresponding samples; temperature elevations of 12.89°C, 35.16°C, and 79.51°C were achieved for 0, 25, and 50 μg/mL GoldMag. Without photothermal ablation, the cell proliferation percentage changed from 100% to 71.3% and 47.0% for cells treated with 25 and 50 μg/mL GoldMag. Photothermal ablation of PANC-1 cells demonstrated an effective treatment response, specifically a reduction to only 61%, 21.9%, and 2.3% cell proliferation for cells treated with 0, 25, and 50 μg/mL GoldMag. MRI was able to visualize GoldMag uptake within PANC-1 cells. Our findings suggest that photothermal ablation may be effective in the treatment of pancreatic cancer. GoldMag nanoparticles could serve as photothermal sensitizers, and MRI is feasible to quantify delivery.