Gas chromatographic-mass spectrometric assay of serotonin in rat superior cervical ganglia. Effects of nerve growth factor and 6-hydroxydopamine
Gas chromatographic-mass spectrometric assay of serotonin in rat superior cervical ganglia. Effects of nerve growth factor and 6-hydroxydopamine
复制标题
大鼠颈上神经节血清素的气相色谱-质谱分析。
DOI：
10.1016/0006-8993(77)90771-5
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
I. Kopin
中科院分区：
文献类型：
--
作者：
A. Liuzzi;F. H. Foppen;J. Saavedra;Rita Levi;I. Kopin
从小鼠唾液腺和其他来源分离的神经生长因子(NGF)对交感神经元的生长有显著的影响8。组织化学研究表明，新生大鼠出生后用NGF-1处理后，包括颈上神经节和虹膜在内的几个组织中5-羟色胺样荧光细胞的数量显著增加。尽管在神经节中有一种脱羧酶表明交感神经节中存在5-羟色胺，但已知芳香氨基酸脱羧酶是一种相对非特异性的酶1。因此，酶活性不能被认为是这种吲哚胺存在的最终证据。本研究用高特异性的5-羟色胺气相色谱-质谱法测定了大鼠颈上神经节和虹膜5-羟色胺的含量。在美国国立卫生研究院进行的实验中，怀孕的斯普拉格-道利大鼠(Zivic-Miller，Allison Park，PA.)在怀孕的第一天获得，并被关在单独的笼子里，直到幼崽出生。新生仔鼠出生后前三天每天皮下注射含牛血清白蛋白(5/zg/g)、神经生长因子(5/zg/g)或6-羟基多巴胺(50#g/g)的1号生理盐水，或同时注射NGF和6-羟基多巴胺。
Nerve growth factor (NGF) isolated from mouse salivary gland as well as from other sources has striking effects on sympathetic neuronal growth 8. Histochemical studies have shown that there is a marked numerical increase in serotonin-like fluorescent cells in several tissues including the superior cervical ganglia and the iris of neonatal rats, when treated since birth with NGF 1. Although the presence of serotonin in sympathetic ganglia has been suggested by the demonstration in the ganglia of an enzyme that decarboxylates tryptophan 5, it is known that aromatic amino acid decarboxylase is a relatively non-specific enzyme 1. Therefore the enzymatic activity could not be considered final proof" for the presence of this indoleamine. In the present study a highly specific gas chromatographic-mass spectrometric assay for serotonin has been used to determine its levels in rat superior cervical ganglia and irises. The biosynthetic enzyme 7 required for its formation was also measured.In experiments performed at the NIH, pregnant Sprague-Dawley rats (Zivic-Miller, Allison Park, Pa.) were obtained on the lgth day of gestation and kept in individual cages until the litters were born. During the first three days of life the newborn pups were injected subcutaneously daily with 50# 1 saline containing bovine albumin (5/zg/g), NGF (5/zg/g) or 6-hydroxydopamine (50# g/g) or both NGF and 6-hydroxydopamine.