Short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine
Short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine
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DOI：
10.1093/jn/129.5.942
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发表时间：
1999-05-01
影响因子：
4.2
通讯作者：
Kiriyama, S
中科院分区：
文献类型：
--
作者：
Hara, H;Haga, S;Kiriyama, S
我们之前的研究表明，摄入短链脂肪酸（SCFA）混合物后，血浆胆固醇水平降低，该混合物由醋酸、丙酸和丁酸钠盐组成，模拟甜菜纤维（SBF）的盲肠发酵产物。在本研究中，我们研究了肝脏和小肠胆固醇合成是否参与了SCFA和SBF的降胆固醇作用。在体外（实验1）和体内（实验2）对大鼠进行了三个单独的实验，研究了胆固醇合成率和门脉血浆中短链脂肪酸浓度的日变化规律（实验3），这些大鼠分别饲喂短链脂肪酸混合物、SBF（100 g/kg日粮）和无纤维对照日粮。用(H2O)-H-3作为示踪剂测定胆固醇合成。体外胆固醇合成率，用肝片测量，SBF组比无纤维对照组高，而SCFA组没有。相比之下，体内肝脏胆固醇合成率在SCFA组比对照组低，但在SBF组没有。全小肠粘膜胆固醇合成率为
We previously showed that plasma cholesterol levels decreased following ingestion of a short-chain fatty acid (SCFA) mixture composed of sodium salts of acetic, propionic, and butyric acids simulating cecal fermentation products of sugar-beet fiber (SBF). In the present study, we investigated whether hepatic and small intestinal cholesterol synthesis is involved in the cholesterol-lowering effects of SCFA and SBF. In vitro (expt. 1) and in vivo (expt. 2) cholesterol synthesis rates and the diurnal pattern of SCFA concentrations in portal plasma (expt. 3) were studied in three separate experiments in rats fed diets containing the SCFA mixture, SBF(100 g/kg diet), or the fiber-free control diet. Cholesterol synthesis was measured using (H2O)-H-3 as a tracer. The in vitro rate of cholesterol synthesis, measured using liver slices, was greater in the SBF group, but not in the SCFA group, than in the fiber-free control group. In contrast, the hepatic cholesterol synthesis rate in vivo was lower in the SCFA group, but not in the SBF group, than in the control group. The mucosal cholesterol synthesis rate for the whole small intestine was