In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds
In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds
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DOI：
10.1093/jxb/eri200
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发表时间：
2005-08-01
影响因子：
6.9
通讯作者：
Thompson, R
中科院分区：
文献类型：
--
作者：
Abirached-Darmency, M;Abdel-gawwad, MR;Thompson, R
种子由几层特殊的细胞类型组成，它们在时间和空间上遵循高度调节的程序进行分裂和分化。采用细胞学方法研究了模式豆科植物蒺藜苜蓿和豌豆胚胎发生中期的组织分化。关于苜蓿属植物种子发育的报道很少。本研究的观察结果揭示了苜蓿种子发育和分化的一些显著特征。转移细胞，参与营养转移到胚胎，被清楚地确定在薄壁组织的最内层珠被。组织学希夫-萘酚使碳水化合物的积累，随后在不同的种子室，并揭示了存储蛋白体。非放射性mRNA原位杂交，进行了使用mRNA探针从两个高度表达的基因编码的主要豌豆球蛋白和豆球蛋白A存储蛋白类型。mRNA表达的时间与已经鉴定的相应蛋白质的时间相关。
The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Transfer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.