A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis
A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis
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DOI：
10.1007/s10529-012-1100-7
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发表时间：
2013-03-01
影响因子：
2.7
通讯作者：
Tyagi, Akhilesh K.
中科院分区：
文献类型：
--
作者：
Khurana, Reema;Kathuria, Hitesh;Tyagi, Akhilesh K.
通过阶段特异性cDNA文库的差异筛选，从水稻（Oryza sativa）花序特异性cDNA文库中克隆到了果胶甲酯酶抑制剂（pecticmethylesteraseinhibitor，OSIPP 3）基因。OSIPP 3作为单拷贝基因存在于水稻基因组中。OSIPP 3基因只在水稻授粉前的小穗中表达。分离了OSIPP 3的上游调控区（URR），并在GUS报告基因上游克隆了一系列5 ′-缺失片段，用于转化拟南芥。OSIPP3_del1和del 2转基因植株在根、花药和角果中均有GUS表达，而OSIPP3_del3转基因植株仅在花药和角果中有GUS活性。在含有OSIPP3_del4构建体的植物的情况下观察到花粉特异性表达。因此，可以得出结论，在-178和+108 bp之间的OSIPP 3 URR对于在拟南芥中赋予花粉特异性表达是必需的。
OSIPP3 gene (coding for pectin methylesterase inhibitor protein) was isolated from a pre-pollinated inflorescence-specific cDNA library by differential screening of stage-specific libraries from Oryza sativa. OSIPP3 is present in the genome of rice as a single copy gene. OSIPP3 gene was expressed exclusively in the pre-pollinated spikelets of rice. Upstream regulatory region (URR) of OSIPP3 was isolated and a series of 5'-deletions were cloned upstream of GUS reporter gene and were used to transform Arabidopsis. OSIPP3_del1 and del2 transgenic plants showed GUS expression in root, anther and silique, while OSIPP3_del3 showed GUS activity only in anthers and siliques. Pollen-specific expression was observed in case of plants harboring OSIPP3_del4 construct. It can, therefore, be concluded that the OSIPP3 URR between -178 and +108 bp is necessary for conferring pollen-specific expression in Arabidopsis.