Gene cloning and expression of a bone morphogenetic protein derived from a murine osteosarcoma.
Gene cloning and expression of a bone morphogenetic protein derived from a murine osteosarcoma.
复制标题
源自鼠骨肉瘤的骨形态发生蛋白的基因克隆和表达。
DOI：
--
复制
发表时间：
1993
影响因子：
4.2
通讯作者：
Hiroshi Nakazatio
中科院分区：
文献类型：
--
作者：
Kunio Takaoka;Hideki Yoshikawa;Jun Hasimoto;K. Masuhara;S. Miyamoto;Shozo Suzuki;Keiro Ono;Masashi Matsui;Shinzo Oikawa;Nobuo Tsuruoka;Yasunori Tawaragi;Chikako Inuzuka;T. Katayama;Masako Sugiyama;Masafumi Tsujimoto;Toshihiro Nakanishi;Hiroshi Nakazatio
根据从小鼠骨肉瘤（Dunn型）中纯化的具有骨诱导活性的蛋白质的部分氨基酸序列信息，筛选肉瘤的cDNA文库以克隆与该蛋白质互补的基因。将克隆的cDNA扩增后转染到中国仓鼠卵巢（CHO）细胞中进行表达。将转染细胞系产生的蛋白与纯载体胶原蛋白一起植入同种异体小鼠体内，两周内植入部位持续出现异位小骨。该cDNA的核苷酸序列及其推导出的氨基酸序列与人骨形态发生蛋白-4（也称为BMP-2B）同源。此外，cDNA和推导出的氨基酸序列与从正常小鼠胎儿中提取的小鼠BMP-4相同。假设克隆的cDNA编码负责失活的dunn型骨肉瘤组织或细胞植入诱导骨形成的蛋白质。该蛋白产物被鉴定为小鼠BMP-4，是tgf - β基因家族的一员。
Based on information from partial amino acid sequences of a protein with bone-inducing activity that was purified from a murine osteosarcoma (Dunn type), a cDNA library of the sarcoma was screened to clone a gene complementary to the protein. The cloned cDNA was amplified and transfected into Chinese hamster ovarian (CHO) cells for expression. When the protein produced by the transfected cell line was implanted in combination with pure carrier collagen into allogeneic mice, ectopic ossicles consistently developed at implanted sites within two weeks. The nucleotide sequence of the cDNA and its deduced amino acid sequence were homologous to those of human bone morphogenetic protein-4 (also BMP-2B). In addition, the cDNA and deduced amino acid sequences were identical to those proposed for murine BMP-4 derived from the normal murine fetus. It is postulated that the cloned cDNA encodes the protein responsible for bone formation induced by implantation of devitalized Dunn-type osteosarcoma tissue or cells. The protein product was identified as murine BMP-4, a member of the TGF-beta gene family.