Mapping Loci that Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato ( Solanum tuberosum L.)
Mapping Loci that Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato ( Solanum tuberosum L.)
复制标题
绘制控制同源四倍体马铃薯 (Solanum tuberosum L.) 中 PVY 引起的块茎和叶部症状的位点
DOI：
10.1101/156539
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Da Silva W
中科院分区：
文献类型：
--
作者：
Da Silva W
马铃薯块茎坏死环斑病（PTNRD）是马铃薯Y病毒（PVYNTN）块茎坏死株感染引起的块茎畸形。PTNRD对块茎质量和适销性产生负面影响，并对全世界的种子和商业马铃薯生产构成严重威胁。pvyntnn症状在Waneta和Pike品种中有所不同：Waneta表达严重的PTNRD和叶面花叶，叶脉和叶面坏死，而Pike不表达PTNRD，花叶是唯一的叶面症状。为了定位影响块茎和叶面症状的位点，用pvyntnny090029分离物接种Waneta和Pike杂交的236个f1后代，使用12808个马铃薯snp进行基因分型。监测叶片症状类型和严重程度10周，同时在收获和储存60 d后再次评估块茎的PTNRD表达。两两相关分析显示PTNRD与静脉坏死之间有很强的相关性（τ = 0.4195）。QTL分析显示，4号和5号染色体对花叶病、4号染色体对PTNRD和5号染色体对叶面坏死症状有主要影响。定位与pvy相关症状相关的QTL为育种者开发可用于消除具有不良表型的马铃薯无性系的标记奠定了基础。即可能发展为PTNRD或无症状的PVY携带者。
Potato tuber necrotic ringspot disease (PTNRD) is a tuber deformity associated with infection by the tuber necrotic strain ofPotato virus Y(PVYNTN). PTNRD negatively impacts tuber quality and marketability, and poses a serious threat to seed and commercial potato production worldwide. PVYNTNsymptoms differ in the cultivars Waneta and Pike: Waneta expresses severe PTNRD and foliar mosaic with vein and leaf necrosis, whereas Pike does not express PTNRD and mosaic is the only foliar symptom. To map loci that influence tuber and foliar symptoms, 236 F1progeny of a cross between Waneta and Pike were inoculated with PVYNTNisolate NY090029 and genotyped using 12,808 potato SNPs. Foliar symptom type and severity were monitored for 10 wk, while tubers were evaluated for PTNRD expression at harvest and again after 60 d in storage. Pairwise correlation analyses indicate a strong association between PTNRD and vein necrosis (τ = 0.4195). QTL analyses revealed major-effect QTL on chromosomes 4 and 5 for mosaic, 4 for PTNRD, and 5 for foliar necrosis symptoms. Locating QTL associated with PVY-related symptoms provides a foundation for breeders to develop markers that can be used to eliminate potato clones with undesirable phenotypes,e.g., those likely to develop PTNRD or to be symptomless carriers of PVY.