Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male × Holstein female).
Effect of nutrient intake on intramuscular glucose metabolism during the early growth stage in cross-bred steers (Japanese Black male × Holstein female).
复制标题
杂交公牛（日本黑公牛×荷斯坦母牛）生长早期营养摄入对肌内葡萄糖代谢的影响。
DOI：
10.1111/j.1439-0396.2012.01310.x
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发表时间：
2013
影响因子：
2.7
通讯作者：
J. Wegner and T. Gotoh.
中科院分区：
文献类型：
--
作者：
Ebara F.;S. Inada;M. Morikawa;S. Asaoka;Y. Isozaki;A. Saito;T. Etoh;Y. Shiotsuka;S. G. Roh ;J. Wegner and T. Gotoh.
目的是探讨生长早期营养摄入对杂交牛骨骼肌糖代谢相关基因表达的影响。从 1.5 至 5 个月龄，H 组 (n= 7) 动物集中饲喂高蛋白和低脂代乳品 [粗蛋白 (CP) 28%；乙醚提取物（EE）18%；最大：2.0 kg，12 l/天]，R组（n= 7）动物喂食有限量的正常代乳粉（CP 25%；EE 23%；最大0.5 kg，4 l/天）。 6～10月龄期间，H组牛饲喂以谷物饲料为主的高营养全混合日粮，R组牛仅饲喂粗饲料。在 3 个活检时间（1.5、5 和 10 月龄）从每只动物身上采集血样，并分析血浆中葡萄糖和胰岛素的浓度。葡萄糖浓度无显着差异；然而，在 5 个月和 10 个月龄时，H 组的胰岛素浓度高于 R 组。通过活检从 1.5、5 和 10 个月龄的胸最长肌 (LT) 中采集肌肉样本。我们使用定量实时聚合酶链反应 (PCR) 检测葡萄糖转运蛋白（GLUT1 和 GLUT4）、胰岛素受体、磷脂酰肌醇 3 激酶 (PI-3K)、蛋白激酶 B (PKB，也称为 Akt)、己糖激酶 1 (HK1) 和肿瘤坏死因子 α (TNFα) 来分析 mRNA 表达水平。虽然在 1.5 和 5 月龄时没有检测到差异，但在 10 月龄时，H 组的 GLUT1、HK1 和 TNFα mRNA 表达水平显着高于 R 组。这些结果表明，在牛 LT 中，影响胰岛素独立介导的葡萄糖摄取的 Glut1 比胰岛素依赖介导的葡萄糖摄取的 GLUT4 对早期生长阶段的营养改善更敏感。
The objective was to investigate the impact of nutrient intake during the early growth period on the expression of glucose metabolism‐related genes in skeletal muscle of cross‐bred cattle. From 1.5 to 5 months of age, group H (n= 7) animals were intensively fed a high‐protein and low‐fat milk replacer [crude protein (CP) 28%; ether extracts (EE) 18%; max: 2.0 kg, 12 l/day], and group R (n= 7) animals were fed a restricted amount of normal milk replacer (CP 25%; EE 23%; max 0.5 kg, 4 l/day). From 6 to 10 months of age, group H cattle were fed a high‐nutrition total mixed ration mainly prepared from grain feed, and group R cattle were fed only roughage. Blood samples were taken from each animal at three biopsy times (1.5, 5 and 10 months of age), and the blood plasma concentration of glucose and insulin was analysed. In glucose concentration, there were no significant differences; however, the concentrations of insulin were higher in group H than in group R at 5 and 10 months of age. Muscle samples were taken by biopsy fromlongissimus thoracismuscle (LT) at 1.5, 5 and 10 months of age. We analysed mRNA expression levels using the quantitative real‐time polymerase chain reaction (PCR) assay for glucose transporters (GLUT1 and GLUT4), insulin receptor, phosphatidylinositol 3‐kinase (PI‐3K), protein kinase B (PKB, also known as Akt), hexokinase 1 (HK1) and tumour necrosis factor alpha (TNFα). Although no differences were detected at 1.5 and 5 months of age, at 10 months of age, GLUT1, HK1 and TNFα mRNA expression levels were significantly higher in group H than in group R. These results suggested Glut1 that affects insulin‐independently mediated glucose uptake was more responsive to improved nutrition during early growth stage than GLUT4 that insulin‐dependently mediated glucose uptake in LT of cattle.