Control of Nucleation Rate for Tetragonal Hen-Egg White Lysozyme Crystals by Application of an Electric Field with Variable Frequencies
Control of Nucleation Rate for Tetragonal Hen-Egg White Lysozyme Crystals by Application of an Electric Field with Variable Frequencies
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DOI：
10.1021/cg801315p
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发表时间：
2009-05-01
影响因子：
3.8
通讯作者：
Uda, S.
中科院分区：
文献类型：
--
作者：
Koizumi, H.;Fujiwara, K.;Uda, S.
控制成核速率对于高质量蛋白质晶体的生长是重要的;因此，需要开发改进的生长方法。外加电场晶体生长是控制成核速率的一种有吸引力的方法。然而，主要是成核率的下降时，观察到的外部电场施加使用直流电压。通过关注液体和固体的化学势，通过施加AC电场成功地实现了成核速率的增加和减少的控制。本文报道了在不同频率的电场作用下，鸡卵白色溶菌酶晶体成核速率的增强和延迟控制，该频率由鸡卵白色溶菌酶溶液和晶体的介电常数决定。
Control of the nucleation rate is important for the growth of high quality protein crystals; therefore, the development of improved growth methods is required. Crystal growth with an external electric field is an attractive method for control of the nucleation rate. However, mainly a decrease in nucleation rate was observed when an external electric field was applied using DC voltage. By focusing on the chemical potential of the liquid and solid, control of both the increase and the decrease in nucleation rate was successfully achieved by applying an AC electric field. Control of nucleation rate in terms of enhancement and retardation is reported for hen-egg white lysozyme crystals by imposition of an electric field with varying frequencies, which was determined by the electrical permittivity of both the hen-egg white lysozyme solution and crystals.