Molecular monitoring of the microbial dynamics occurring on historical limestone buildings during and after the in situ application of different bio-consolidation treatments.
Molecular monitoring of the microbial dynamics occurring on historical limestone buildings during and after the in situ application of different bio-consolidation treatments.
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DOI：
10.1016/j.scitotenv.2011.08.063
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发表时间：
2011-11-15
期刊：
影响因子：
--
通讯作者：
Jroundi F
中科院分区：
文献类型：
--
作者：
Ettenauer J;Piñar G;Sterflinger K;Gonzalez-Muñoz MT;Jroundi F
微生物诱导碳酸盐沉淀法是一种环境友好的观赏石腐朽保护方法，被引入文化遗产保护领域。最近的保护研究在实验室条件下进行的非无菌钙屑砂岩石头已成功地报告了一个合适的营养液，接种和非接种粘球菌xanthus，作为生物固结处理的应用程序。此外，这一程序已被应用于现场，最近，在西班牙格拉纳达选定的历史建筑。我们第一次评估了在格拉纳达的两座历史建筑上原位应用上述处理方法的效率和风险。评估包括详细调查在7天的治疗期间活跃生长的微生物群-短期监测和在应用6个月和12个月后保留在石头上的微生物群-长期监测。采用分子策略，包括DNA提取、16 S rRNA序列的PCR扩增、克隆库的构建以及通过变性梯度凝胶电泳（DGGE）分析进行指纹图谱分析，然后进行测序，以深入了解差异处理的石头上存在的微生物多样性。对M.通过使用种特异性引物的PCR进行xanthus。通过应用营养溶液（接种或未接种），在两个建筑物上触发了相似的动力学。16 S rDNA测序显示，在7天的治疗期间，属于厚壁菌门和变形菌门的成员占主导地位，而一年后，厚壁菌门的芽孢杆菌目是主要检测到的微生物。M.仅在处理的7天期间可以检测到黄曲霉。这些处理似乎没有激活危险的微生物，而且，在很长一段时间后，在结石上选择了一组同质的碳酸化细菌的剩余部分。两座历史建筑接受了两种不同的原位生物加固处理。③采用分子生物学技术对微生物群落进行监测。在短期和长期监测期间，建筑物上发生了类似的动态。③处理改变了原始异质微生物区系。建立了产碳酸盐细菌的均一微生物群（主要是厚壁菌门）。
Microbially Induced Carbonate Precipitation is proposed as an environmentally friendly method to protect decayed ornamental stone and introduced in the field of preservation of Cultural Heritage. Recent conservation studies performed under laboratory conditions on non-sterile calcarenite stones have successfully reported on the application of a suitable nutritional solution, inoculated and non-inoculated with Myxococcus xanthus, as a bioconsolidation treatment. Furthermore, this procedure has been applied in situ, very recently, to selected historical buildings in Granada, Spain. For the first time, we evaluate the efficiency and risks of the in situ application of the above mentioned treatments onto two historical buildings in Granada. The evaluation consists of a detailed investigation of the micro-biota actively growing during the seven days of the treatments – short-term monitoring and of that remaining on the stones after six and twelve months of the application – long-term monitoring. A molecular strategy, including DNA extraction, PCR amplification of 16S rRNA sequences, construction of clone libraries and fingerprinting by DGGE (Denaturing Gradient Gel Electrophoresis) analysis followed by sequencing was used to gain insight into the microbial diversity present on the differentially treated stones. The monitoring of M. xanthus was performed by PCR using species-specific primers. Similar dynamics were triggered on both buildings by the application of the nutritional solution (inoculated or non-inoculated). 16S rDNA sequencing revealed the dominant occurrence of members belonging to the Firmicutes and Proteobacteria during the seven days of the treatment, whereas after one year the order Bacillales of the phylum Firmicutes was the predominantly detected microorganisms. M. xanthus could be detected only during the seven days of the treatment. The treatments seem to activate no dangerous microorganisms and furthermore, to select the remainder of a homogeneous group of carbonatogenic bacteria on the stones after a long period of time. ► Two historical buildings received two different in situ bioconsolidation treatments. ► The microbial communities were monitored with molecular techniques. ► Similar dynamics occurred on the buildings during a short- and long-time monitoring. ► The primordial heterogeneous micro-biota was changed by the treatments. ► A homogenous micro-biota of carbonatogenic bacteria was established (mainly Firmicutes).
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影响因子：
2.8
作者：
Castanier, S;Le Métayer-Levrel, G;Perthuisot, JP
通讯作者：
Perthuisot, JP
影响因子：
2.2
作者：
Schabereiter-Gurtner, C;Piñar, G;Rölleke, S
通讯作者：
Rölleke, S
影响因子：
8.8
作者：
Jimenez-Lopez, C.;Rodriguez-Navarro, C.;Gonzalez-Munoz, M. T.
通讯作者：
Gonzalez-Munoz, M. T.
影响因子：
3.6
作者：
Pinar, Guadalupe;Jimenez-Lopez, Concepcion;Sterflinger, Katja;Ettenauer, Joerg;Jroundi, Fadwa;Fernandez-Vivas, Antonia;Teresa Gonzalez-Munoz, Maria
通讯作者：
Teresa Gonzalez-Munoz, Maria
影响因子：
4.4
作者：
Rodriguez-Navarro, C;Rodriguez-Gallego, M;Gonzalez-Muñoz, MT
通讯作者：
Gonzalez-Muñoz, MT