Malignant transformation of erythroid cells in vivo by introduction of a nonreplicating retrovirus vector.
Malignant transformation of erythroid cells in vivo by introduction of a nonreplicating retrovirus vector.
复制标题
通过引入非复制逆转录病毒载体对体内红系细胞进行恶性转化。
DOI：
10.1126/science.2990034
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发表时间：
1985
期刊：
影响因子：
56.9
通讯作者：
S. Ruscetti
中科院分区：
文献类型：
--
作者：
L. Wolff;S. Ruscetti
从复制缺陷型脾病灶形成病毒（SFFV）的DNA重建和转染到psi-2细胞含有包装缺陷型突变的莫洛尼鼠白血病病毒。无复制能力的逆转录病毒颗粒（无辅助病毒，含有表达SFFV（gp 52）的转化包膜基因的基因组）在体外转化骨髓细胞，并且在直接静脉内引入载体后，在体内诱导恶性红系疾病。疾病的诱导依赖于预先用苯肼处理小鼠，这可能增加了红系靶细胞的可用性。由于没有证据表明病毒颗粒在患有恶性疾病的小鼠中表达，因此本研究证明了有限数量的表达SFFV gp 52的红系细胞的急性致癌潜力。用含有转化基因的非复制型逆转录病毒载体直接接种动物可能有助于研究这些基因的致癌作用。
DNA from a replication-defective spleen focus-forming virus (SFFV) was reconstructed and transfected into psi-2 cells containing a packaging-defective mutant of Moloney murine leukemia virus. Replication-incompetent retrovirus particles (helper virus-free containing genomes that express the transforming envelope gene of SFFV (gp52) transformed bone marrow cells in vitro and, after direct intravenous introduction of the vector, induced malignant erythroid disease in vivo. Disease induction was dependent on prior treatment of mice with phenylhydrazine, which probably increased the availability of erythroid target cells. Since there was no evidence of virus particle expression in mice with malignant disease, this study demonstrates the acute oncogenic potential of a limited number of erythroid cells expressing SFFV gp52. Direct inoculation of animals with nonreplicating retroviral vectors containing transforming genes may be useful in study the oncogenic effects of such genes.
弗兰德病毒对造血细胞进行体外红系转化不需要辅助病毒。
DOI：
10.1073/pnas.77.9.5287
发表时间：
1980
影响因子：
11.1
作者：
Hankins,WD;Krantz,SB
通讯作者：
Krantz,SB