Monoclonal antibodies to novel myeloid antigens reveal human neutrophil heterogeneity.
Monoclonal antibodies to novel myeloid antigens reveal human neutrophil heterogeneity.
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针对新型骨髓抗原的单克隆抗体揭示了人类中性粒细胞的异质性。
DOI：
10.1073/pnas.79.17.5374
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发表时间：
1982
影响因子：
11.1
通讯作者：
Fanger,MW
中科院分区：
文献类型：
--
作者：
Ball,ED;Graziano,RF;Shen,L;Fanger,MW
三种细胞毒性小鼠单克隆抗体识别髓细胞特异性抗原已产生免疫正常人中性粒细胞或髓母细胞从急性髓单细胞白血病患者。其中两种，pmn6和pmn29，对中性粒细胞有特异性；第三种单克隆抗体AML-2-23与大多数正常单核细胞以及成熟中性粒细胞亚群有反应。尽管所有个体的中性粒细胞都表达这些抗原，但细胞荧光分析显示，携带pmn6和AML-2-23抗原的细胞百分比在个体之间有所不同。在携带抗原的细胞中，每种抗原的密度也有显著的异质性。这三种抗体均能有效介导急性髓细胞白血病细胞的补体依赖性细胞毒性，但对淋巴细胞白血病细胞无反应。中性粒细胞的细胞毒性由pmn6和pmn29介导，AML-2-23不参与。另一方面，AML-2-23对正常单核细胞和巨噬细胞具有细胞毒性，而pmn6和pmn29不具有细胞毒性。这些单克隆抗体可能在正常中性粒细胞功能和分化的研究中有价值，并可能在髓性白血病的诊断和治疗中具有临床应用价值。
Three cytotoxic murine monoclonal antibodies that recognize myeloid-specific antigens have been produced by immunization with normal human neutrophils or myeloblasts from a patient with acute myelomonocytic leukemia. Two of these, PMN 6 and PMN 29, are specific for neutrophils; the third monoclonal antibody, AML-2-23, is reactive with the majority of normal monocytes as well as a subpopulation of mature neutrophils. Although neutrophils from all individuals tested expressed these antigens, cytofluorographic analysis revealed that the percentage of cells bearing the PMN 6 and AML-2-23 antigens varied among individuals. Significant additional heterogeneity in the density of each antigen among antigen-bearing cells was also observed. All three antibodies efficiently mediated complement-dependent cytotoxicity of acute myelocytic leukemia cells yet were unreactive with lymphocytic leukemia cells. Neutrophil cytotoxicity was mediated by PMN 6 and PMN 29 but not by AML-2-23. On the other hand, AML-2-23, but not PMN 6 or PMN 29, was cytotoxic for normal monocytes and macrophages. These monoclonal antibodies may be of value in the study of normal neutrophil function and differentiation and may have clinical utility in diagnosis and therapy of myeloid leukemia.