THE EXPRESSION AND ROLES OF INHIBITOR OF DNA BINDING HELIX-LOOP-HELIX PROTEINS IN THE DEVELOPING AND ADULT MOUSE RETINA
THE EXPRESSION AND ROLES OF INHIBITOR OF DNA BINDING HELIX-LOOP-HELIX PROTEINS IN THE DEVELOPING AND ADULT MOUSE RETINA
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DOI：
10.1016/j.neuroscience.2010.12.007
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发表时间：
2011-02-17
期刊：
影响因子：
3.3
通讯作者：
Yip, H. K.
中科院分区：
文献类型：
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作者：
Du, Y.;Yip, H. K.
DNA结合抑制剂（Id）蛋白结合并抑制碱性螺旋-环-螺旋（bHLH）转录因子的功能，包括调节视网膜发育的转录因子。然而，很少有人知道Id蛋白在视网膜发育过程中的生长和分化的作用。本研究的目的是观察Id蛋白在发育和成年小鼠视网膜中的表达，作为研究Id家族成员在眼睛中功能的第一步。通过实时定量PCR、Western blot和免疫组织化学方法检测野生型和Id 1/Id 3双敲除小鼠中Id 1 -4的表达。Id 1 -4基因和蛋白在胚胎期和出生后早期视网膜中表达水平较高，而在成年期表达水平下降。在胚胎（E）第13.5天至16.5天的内成神经细胞层（NBL）中观察到Id蛋白的表达。Id 4表达开始于E18.5。到E18.5和出生后第1天，Id 1 -4的表达在神经节细胞层和NBL内部表现出明显但重叠的模式。在成人中，Ids在视网膜神经节细胞、无长突细胞、双极细胞和水平细胞中表达。在Muller细胞中未发现Id表达。Id 1和Id 3双基因敲除小鼠（Id 1（-/-）/Id 3（-/-））与野生型或杂合子同窝小鼠相比，显示出较小的视网膜尺寸。然而，在Id 1和Id 3单敲除视网膜的组织学分析显示没有明显的缺陷，在发育表型。我们的研究结果表明，Id家族的表达可能在调节视网膜祖细胞增殖和分化中发挥重要作用。(c)2011年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Inhibitor of DNA binding (Id) proteins bind to and inhibit the function of basic helix-loop-helix (bHLH) transcription factors including those that regulate retinal development. However, little is known about the role of Id proteins in the growth and differentiation of the retina during development. The purpose of this study is to observe the expression of Id proteins in the developing and adult mouse retinas as the first step in investigating the functions of Id family members in the eye. The expression of Id1-4 was examined by real-time PCR, Western blot, and immunohistochemistry in wild-type and Id1/Id3 double-knockout mice. Id1-4 genes and proteins showed high expression levels in the retina at embryonic and early postnatal stages, whereas declined in the adult. Expression of Id proteins was observed in the inner neuroblastic layer (NBL) at embryonic (E) day 13.5 through 16.5. Id4 expression began at E18.5. By E18.5 and postnatal day 1, the expression of Id1-4 exhibited distinct yet overlapping patterns in the ganglion cell layer and inner part of NBL. In the adult, Ids were expressed in retinal ganglion cells, amacrine cells, bipolar cells, and horizontal cells. No Id expression was found in Muller cells. Id1 and Id3 double-knockout mice (Id1(-/-)/Id3(-/-)) showed smaller retinal size compared to wild-type or heterozygous littermates. However, histological analyses in Id1 and Id3 single-knockout retinas revealed no obvious defects in developmental phenotype. Our results indicate that the expression of the Id family may play an important role in regulating retinal progenitor cell proliferation and differentiation. (c) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.