Preventive effect of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) against endotoxin-induced muscle atrophy
Preventive effect of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) against endotoxin-induced muscle atrophy
复制标题
二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）对内毒素引起的肌肉萎缩的预防作用
DOI：
10.1016/j.clnesp.2021.07.017
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发表时间：
2021
期刊：
影响因子：
3
通讯作者：
Fujino H
中科院分区：
文献类型：
--
作者：
Yamaguchi A;Nishida Y;Maeshige N;Moriguchi M;Uemura M;Ma X;Miyoshi M;Kondo H;Fujino H
背景与目的肌肉萎缩是一个公共卫生问题，炎症是肌肉萎缩的主要原因。虽然二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）是典型的ω-3多不饱和脂肪酸，据报道对内毒素诱导的炎症反应具有抗炎作用，但其对炎性肌肉萎缩的作用尚未阐明。方法将25、50、100 μM的DHA或EPA分别加入C2 C12肌管中，1h后加入1 μg/mL的脂多糖（LPS）。第一次加入LPS后2小时，测量C2 C12肌管中atrogin-1和Murf-1的mRNA表达。在第一次LPS添加后24小时进行第二次LPS添加，并测量肌管直径、肌原纤维蛋白和细胞活力。单因素方差分析和Tukey的多重比较检验用于统计处理的结果，和显着性水平被设置为小于5%.ResultsThe LPS添加组显着降低肌管直径和肌原纤维蛋白含量相比，对照组。与LPS组相比，25 μM、50 μM DHA和25 μM EPA添加组的肌管直径显著更大。添加25 μM DHA和EPA组的肌原纤维蛋白含量显著高于LPS组。与LPS组相比，添加25 μM DHA和EPA的组中atrogin-1和murf-1的mRNA表达水平被显著抑制。细胞活力没有改变，通过添加LPS，DHA，和EPA.ConclusionsThe除了DHA或EPA抑制肌管直径和肌原纤维蛋白含量的下降，并抑制atrogin-1和murf-1的增加由LPS诱导。这项研究表明DHA和EPA对内毒素诱导的肌肉萎缩有预防作用。
Background & aimsMuscle atrophy is a public health issue and inflammation is a major cause of muscle atrophy. While docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are typical ω-3 polyunsaturated fatty acids, are reported to have anti-inflammatory effects on endotoxin-induced inflammatory responses, their effects on inflammatory muscle atrophy have not been clarified. In this study, we aimed to investigate the effects of DHA and EPA on inflammatory muscle atrophy.MethodsDHA or EPA was added to C2C12 myotubes at a concentration of 25, 50, or 100 μM, and 1 h later, lipopolysaccharide (LPS) was added at a concentration of 1 μg/mL. Two hours after the first LPS addition, mRNA expression of atrogin-1 and Murf-1 in C2C12 myotubes was measured. The second LPS addition was performed 24 h after the first LPS addition, and myotube diameter, myofibrillar protein, and cell viability were measured. One-way ANOVA and Tukey's multiple comparison test were used for statistical processing of the results, and the significance level was set to less than 5 %.ResultsThe LPS-added group significantly decreased the myotube diameter and the myofibrillar protein content compared to the control group. The myotube diameter was significantly higher in the 25 μM, 50 μM DHA and 25 μM EPA-added groups compared to the LPS group. In the 25 μM DHA and EPA-added groups, the myofibrillar protein content was significantly higher than that in the LPS group. The mRNA expression levels of atrogin-1 and murf-1 were significantly suppressed in the 25 μM DHA and EPA-added groups compared to the LPS group. The cell viability did not change by the addition of LPS, DHA, and EPA.ConclusionsThe addition of DHA or EPA suppressed the decrease in myotube diameter and myofibrillar protein content and suppressed the increase in atrogin-1 and murf-1 induced by LPS. This study showed the preventive effect of DHA and EPA on endotoxin-induced muscle atrophy.