Preparation, characterisation and maintenance of drug efficacy of doxorubicin-loaded human serum albumin (HSA) nanoparticles
Preparation, characterisation and maintenance of drug efficacy of doxorubicin-loaded human serum albumin (HSA) nanoparticles
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DOI：
10.1016/j.ijpharm.2007.03.036
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发表时间：
2007-08-16
影响因子：
5.8
通讯作者：
Langer, K.
中科院分区：
文献类型：
--
作者：
Dreis, S.;Rothweller, F.;Langer, K.
人血清白蛋白（HSA）纳米颗粒是很有前途的药物载体系统。细胞抑制剂与HSA纳米颗粒结合可降低其毒性，优化其体内分布和/或克服多药耐药。在本研究中，制备了负载阿霉素的HSA纳米颗粒制剂。阿霉素通过吸附到纳米颗粒表面或通过掺入到颗粒基质中被装载到HSA纳米颗粒上。两种负载策略均可获得尺寸在150 ~ 500 nm之间的HSA纳米颗粒，负载效率为70 ~ 95%。在两种不同的神经母细胞瘤细胞系中研究了所得纳米颗粒对细胞活力的影响。与阿霉素溶液相比，载药纳米颗粒的抗癌作用增强。基于这些结果，建立了用于进一步抗肿瘤研究的制备负载阿霉素的HSA纳米颗粒的标准方案。(c) 2007 Elsevier B.V.版权所有
Human serum albumin (HSA) nanoparticles represent promising drug carrier systems. Binding of cytostatics to HSA nanoparticles may diminish their toxicity, optimise their body distribution and/or may overcome multidrug resistance. In the present study, doxorubicin-loaded HSA nanoparticle preparations were prepared. Doxorubicin was loaded to the HSA nanoparticles either by adsorption to the nanoparticles' surfaces or by incorporation into the particle matrix. Both loading strategies resulted in HSA nanoparticles of a size range between 150 nm and 500 nm with a loading efficiency of 70-95%. The influence on cell viability of the resulting nanoparticles was investigated in two different neuroblastoma cell lines. The anti-cancer effects of the drug-loaded nanoparticles were increased in comparison to doxorubicin solution. Based on these result a standard protocol for the preparation of doxorubicin-loaded HSA nanoparticles for further antitumoural studies was established. (c) 2007 Elsevier B.V. All rights reserved.