Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice
Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice
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DOI：
10.1016/j.bbrc.2012.07.081
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发表时间：
2012-08-24
影响因子：
3.1
通讯作者：
Tamiya, Gen
中科院分区：
文献类型：
--
作者：
Jambaldorj, Jamiyansuren;Makino, Satoshi;Tamiya, Gen
TATA盒结合蛋白相关因子1(TAF1)蛋白是RNA聚合酶II介导的基因转录途径中最大的TFIID复合体的重要组成部分，它调控着大量与细胞分裂相关的基因的转录。我们以前报道的神经元特异性TAF1基因亚型(N-TAF1)可能通过对许多神经元特异性基因的转录调控而在神经元中发挥重要作用。在本研究中，我们克隆了编码小鼠N-TAF1(N-TAF1)蛋白同源物的全长cDNA。通过实时荧光定量RT-PCR，研究了N-TAF1在小鼠组织和细胞系中的表达。与人类N-TAF1一样，N-TAF1在大脑和神经母细胞瘤中表达有限，而TALL在其他地方表达。此外，在小鼠胚胎头部或小鼠脑中，TAF1的mRNA表达在发育过程中变化很大，但N-TAF1的表达持续存在。我们的结果表明，N-TAF1基因在神经发生过程中对神经元细胞的不分裂起重要作用，而不是在细胞分裂和增殖过程中起重要作用。(C)2012 Elsevier Inc.保留所有权利。
TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II-mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N-Taf1 mRNA in mouse tissues and cell lines. As well as the human N-TAF1, the N-Taf1 showed limited expression in the brain and neuroblastoma, whereas Tall expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N-Taf1 showed sustained expression. Our result suggests that the N-Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis. (c) 2012 Elsevier Inc. All rights reserved.