Lipase H, a new member of the triglyceride lipase family synthesized by the intestine
Lipase H, a new member of the triglyceride lipase family synthesized by the intestine
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DOI：
10.1006/geno.2002.6837
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发表时间：
2002-09-01
期刊：
影响因子：
4.4
通讯作者：
Rader, DJ
中科院分区：
文献类型：
--
作者：
Jin, WJ;Broedl, UC;Rader, DJ
我们在此报告了甘油三酯脂肪酶家族新成员的分子克隆，该成员是编码人脂肪酶 H (LIPH) 和小鼠直向同源物 (Liph) 的 2.4-kb cDNA。人类 LIPH cDNA 编码具有脂肪酶结构域的 451 个氨基酸的蛋白质。小鼠 Liph 与人 LIPH 具有 85% 的氨基酸同一性和 75% 的核苷酸同一性。人 LIPH 与磷脂酰丝氨酸特异性磷脂酶 A1 (PS-PLA1) 具有 47% 的同一性，与内皮脂肪酶 (LIPG) 和脂蛋白脂肪酶 (LPL) 具有 46% 的同一性。 LIPH 位于人类染色体 3q27-q28 上。 Northern 印迹分析揭示了 LIPH mRNA 在肠、肺和胰腺中的特异性表达。在人体肠道中也检测到了脂肪酶 H 蛋白。脂肪酶 H 是一种分泌蛋白，表观分子量为 63 kDa。尽管测试了几种脂质底物，但未鉴定出 LIPG 的脂质底物。与该基因家族的其他成员一样，LIPH 可能参与脂质和能量代谢。
We report here the molecular cloning of a novel member of the triglyceride lipase family, a 2.4-kb cDNA encoding human lipase H (LIPH) and the mouse ortholog (Liph). The human LIPH cDNA encodes a 451-amino-acid protein with a lipase domain. Mouse Liph shows 85% amino acid identity and 75% nucleotide identity to human LIPH. Human LIPH exhibits 47% identity with phosphatidylserine-specific phospholipase A1 (PS-PLA1) and 46% identity with endothelial lipase (LIPG) and lipoprotein lipase (LPL). LIPH is localized on human chromosome 3q27-q28. Northern blot analysis revealed specific expression of LIPH mRNA in intestine, lung, and pancreas. Lipase H protein was also detected in human intestine. Lipase H is a secreted protein with an apparent molecular weight of 63 kDa. Although several lipid substrates were tested, the lipid substrate of LIPG was not identified. Like the other members of this gene family, LIPH may be involved in lipid and energy metabolism.