Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC/MS in the NICI mode
Determination of epoxyeicosatrienoic acids in human red blood cells and plasma by GC/MS in the NICI mode
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DOI：
10.1016/j.jchromb.2008.10.035
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发表时间：
2008-12-01
影响因子：
3
通讯作者：
Lucas, Daniele
中科院分区：
文献类型：
--
作者：
Goulitquer, Sophie;Dreano, Yvonne;Lucas, Daniele
环氧二十碳三烯酸（Epoxyeicosatrienoic Acids，ESTs）是花生四烯酸的细胞色素P450代谢产物，参与血管张力的调节。尽管在各种生理效应中，雌二醇的重要性，但很少有方法被开发出来定量它们在人体血液中的含量。这使我们开发了一种负离子化学电离的GC/MS方法。由于雌二醇主要位于磷脂中，因此在固相萃取后用磷脂酶A（2）水解红细胞（RBC）和血浆磷脂。然后，将雌二醇衍生为五氟苄基酯，以[H-2（8）]-花生四烯酸为内标进行定量。发现血浆中的雌二醇浓度为106 +/- 37 ng/mL（-1），RBC中的雌二醇浓度为33.4 +/- 8.5 ng/10（9）（平均值+/- S.D.）10名健康志愿者它们在红细胞中的量是血浆中的3倍：这两个参数被证明具有良好的相关性。(C)2008 Elsevier B. V.保留所有权利。
Epoxyeicosatrienoic acids (EETs) are cytochrome P450 metabolites of arachidonic acid involved in the regulation of vascular tone. Despite the importance of EETs in a variety of physiological effects, few methods have been developed to quantity them in human blood. This led us to develop a method by GC/MS with negative ion chemical ionization. As EETs are primarily located in phospholipids, red blood cells (RBCs) and plasma phospholipids were hydrolyzed with phospholipase A(2) after a solid phase extraction. Then, EETs were derivatized as pentafluorobenzyl esters, and [H-2(8)]-arachidonic acid was used as internal standard for quantification. EETs were found to be at concentrations of 106 +/- 37 ng mL(-1) in plasma and 33.4 +/- 8.5 ng/10(9) RBCs (mean +/- S.D.) in 10 healthy volunteers. Their amount in RBCs was 3-fold that in plasma: both parameters proved to be well correlated. (C) 2008 Elsevier B.V. All rights reserved.