Comparison of expression patterns of keratin 6, 7, 16, 17, and 19 within multiple independent isolates of As(+3)- and Cd (+2)-induced bladder cancer : keratin 6, 7, 16, 17, and 19 in bladder cancer.
Comparison of expression patterns of keratin 6, 7, 16, 17, and 19 within multiple independent isolates of As(+3)- and Cd (+2)-induced bladder cancer : keratin 6, 7, 16, 17, and 19 in bladder cancer.
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As(3) 和 Cd(2) 诱导的膀胱癌的多个独立分离株中角蛋白 6、7、16、17 和 19 表达模式的比较：膀胱癌中的角蛋白 6、7、16、17 和 19
DOI：
10.1007/s10565-010-9169-z
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发表时间：
2011
影响因子：
6.1
通讯作者：
Sens,DonaldA
中科院分区：
文献类型：
--
作者：
Somji,Seema;Cao,Ling;Mehus,Aaron;Zhou,XuDong;Sens,MaryAnn;Dunlevy,JaneR;Garrett,ScottH;Zheng,Yun;Larson,JenniferL;Sens,DonaldA
本实验室通过在相似的暴露条件下将亲代UROtsa细胞暴露于这两种药物，产生了七种镉(Cd+2)和六种亚砷酸盐(As+3)转化的尿路上皮癌细胞系。本研究检测了7株CD+2转化细胞株和6株AS+3细胞株的角蛋白6、16和17的表达，以及6株AS+3细胞株的角蛋白7和19的表达。结果表明，Cd+2转化的细胞系及其各自的移植细胞均表达角蛋白6、16和17的mRNA和蛋白。角蛋白6、16和17的表达也与经历鳞状分化的尿路上皮肿瘤细胞的面积相关。结果还显示，在6个AS+3转化细胞系中，有4个细胞株表达角蛋白7和19的mRNA和蛋白，并产生了角蛋白7和19的局灶性染色；另外2个AS+3转化的细胞株的角蛋白7的mRNA和蛋白的表达很低，产生的皮下肿瘤对角蛋白7没有免疫反应；其中一株细胞所产生的腹膜肿瘤恢复了角蛋白7蛋白的表达。目前的结果，结合以前的研究，表明CD+2或As+3对URotsa细胞的恶性转化在所产生的一系列细胞系及其相应的肿瘤中产生相似的角蛋白6、7、16、17和19模式。
This laboratory has generated a series of seven cadmium (Cd+2)- and six arsenite (As+3)-transformed urothelial cancer cell lines by exposure of parental UROtsa cells to each agent under similar conditions of exposure. In this study, the seven Cd+2-transformed cell lines were characterized for the expression of keratin 6, 16, and 17 while the six As+3cell lines were assessed for the expression of keratin 7 and 19. The results showed that the series of Cd+2-transformed cell lines and their respective transplants all had expression of keratin 6, 16, and 17 mRNA and protein. The expression of keratin 6, 16, and 17 was also correlated with areas of the urothelial tumor cells that had undergone squamous differentiation. The results also showed that four of the six As+3-transformed cell lines had expression of keratin 7 and 19 mRNA and protein and produced subcutaneous tumors with intense focal staining for keratin 7 and 19. The other two As+3-transformed cell lines had very low expression of keratin 7 mRNA and protein and produced subcutaneous tumors having no immunoreactivity for keratin 7; although keratin 19 expression was still present. The peritoneal tumors produced by one of these two cell lines regained expression of keratin 7 protein. The present results, coupled with previous studies, indicate that malignant transformation of UROtsa cells by Cd+2or As+3produce similar patterns of keratin 6, 7, 16, 17, and 19 in the resulting series of cell lines and their respective tumors.