Sub-cellular mRNA localization modulates the regulation of gene expression by small RNAs in bacteria
Sub-cellular mRNA localization modulates the regulation of gene expression by small RNAs in bacteria
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DOI：
10.1088/1478-3975/aa69ac
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发表时间：
2017-03
期刊：
影响因子：
2
通讯作者：
H. Teimouri;Elgin Korkmazhan;J. Stavans;E. Levine
中科院分区：
文献类型：
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作者：
H. Teimouri;Elgin Korkmazhan;J. Stavans;E. Levine
小的非编码RNA可以对细菌中的基因表达发挥重要的调控作用。近年来，通过sRNA理解细菌基因表达已经取得了实质性进展。然而，最近的研究结果表明，家族的mRNA显示出非平凡的亚细胞分布提出了一个问题，如何定位可能会影响调节活性的sRNA。在这里，我们在一个简单的数学模型中解决这个问题。我们发现，mRNA的非均匀空间分布可以改变阈值线性响应，其特征在于sRNA的化学计量作用，并调节由相同sRNA共同调节的靶标之间的层次结构。我们还确定了sRNA途径中辅因子的亚细胞组织可以诱导sRNA靶点空间异质性的条件。我们的研究结果表明，在某些条件下，解释和建模的自然和合成基因调控电路需要考虑到参与基因的转录本的空间组织。
Small non-coding RNAs can exert significant regulatory activity on gene expression in bacteria. In recent years, substantial progress has been made in understanding bacterial gene expression by sRNAs. However, recent findings that demonstrate that families of mRNAs show non-trivial sub-cellular distributions raise the question of how localization may affect the regulatory activity of sRNAs. Here we address this question within a simple mathematical model. We show that the non-uniform spatial distributions of mRNA can alter the threshold-linear response that characterizes sRNAs that act stoichiometrically, and modulate the hierarchy among targets co-regulated by the same sRNA. We also identify conditions where the sub-cellular organization of cofactors in the sRNA pathway can induce spatial heterogeneity on sRNA targets. Our results suggest that under certain conditions, interpretation and modeling of natural and synthetic gene regulatory circuits need to take into account the spatial organization of the transcripts of participating genes.