Cloning and expression analysis of a HSP70 gene from Korean rockfish (Sebastes schlegeli).
Cloning and expression analysis of a HSP70 gene from Korean rockfish (Sebastes schlegeli).
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DOI：
10.1016/j.fsi.2013.07.022
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发表时间：
2013-10
影响因子：
4.7
通讯作者：
W. Mu;H. Wen;Ji-Fang Li;F. He
中科院分区：
文献类型：
--
作者：
W. Mu;H. Wen;Ji-Fang Li;F. He
The gene encoding HSP70 was isolated from Korean rockfishSebastes schlegeliby homologous cloning and rapid amplification of cDNA ends (RACE). The full-length of HSP70 cDNA was composed of 2259 bp and encoded a polypeptide of 639 amino acids. BLAST analysis showed that HSP70 ofS. schlegelishared high identities with those of theLates calcarifer, Oreochromis niloticus,Seriola quinqueradiataHSP70s (88–89%). Our current study also revealed that HSP70 of Korean rockfish was expressed in many tissues by RT-PCR under unstressed condition. Quantitative real-time PCR showed that the expression patterns of Korean rockfish HSP70 were developmental stage-dependency. The expression of HSP70 was measured by quantitative real-time PCR after different oxygen treatments. The results showed that expression of HSP70 increased significantly after exposure to hypoxia for 30 min in gill and ovary, and then decreased for 60 min, and the level in spleen and liver gradually increased and reached the highest at 60 min. In addition, in gill, spleen and liver, the HSP70 mRNA level reached the maximum in hypoxia group after one hour different oxygen concentration stress. Increased amounts of serum thyroxine (T4), and triiodothyronine (T3) were also found during 30 min hypoxia treatment and 60 min normoxia group in our study. All of the results provide information to further study the mechanism of physiology and immune function under stress conditions of ovoviviparous teleosts.
