Ice Nucleation in Emulsified Aqueous Solutions of Antifreeze Protein Type III and Poly(vinyl alcohol)
Ice Nucleation in Emulsified Aqueous Solutions of Antifreeze Protein Type III and Poly(vinyl alcohol)
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DOI：
10.1021/jp111745v
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发表时间：
2011-06-23
影响因子：
3.3
通讯作者：
Seto, Takafumi
中科院分区：
文献类型：
--
作者：
Inada, Takaaki;Koyama, Toshie;Seto, Takafumi
抗冻蛋白（AFP）III和聚乙烯醇（PVA）被称为抗冰成核剂（anti-INA），其抑制异相冰成核。然而，这些抗INAs在抑制油包水（W/O）乳液中的冰成核中的有效性尚不清楚，其中均匀冰成核可以通过实验模拟。在这项研究中，在AFP III，PVA，和其他nonanti-INA聚合物的乳化溶液中的冰成核温度进行了测量，然后根据经典的成核理论的成核率进行了分析。结果表明，冰成核是表面引发的，除了PVA溶液，可能引起的乳化剂，SPAN 65，在液滴表面的非均匀性。在此成核模式下，AFP In对冰核速率没有显著影响。与此相反，PVA表现出的冰成核活性仅在液滴表面，这表明成核是由于PVA和SPAN 65之间的相互作用。
Antifreeze protein (AFP) III and poly(vinyl alcohol) (PVA) are known as anti-ice nucleating agents (anti-INAs), which inhibit heterogeneous ice nucleation. However, the effectiveness of these anti-INAs in inhibiting ice nucleation in water-in-oil (W/O) emulsions, in which homogeneous ice nucleation can be experimentally simulated, is unclear. In this study, the ice nucleation temperature in emulsified solutions of AFP III, PVA, and other nonanti-INA polymers was measured, and then the nucleation rate was analyzed based on classical nucleation theory. Results showed that ice nucleation was surface-initiated and, except for PVA solutions, probably caused heterogeneously by the emulsifier, SPAN 65, at the droplet surfaces. In this nucleation mode, AFP In had no significant effect on the ice nucleation rate. In contrast, PVA exhibited ice-nucleating activity only at the droplet surfaces, suggesting that the nucleation is due to the interaction between PVA and SPAN 65.