How much TCR does a T cell need?
How much TCR does a T cell need?
复制标题
DOI：
10.1016/s1074-7613(01)00170-4
复制
发表时间：
2001-07-01
期刊：
影响因子：
32.4
通讯作者：
Mathis, D
中科院分区：
文献类型：
--
作者：
Labrecque, N;Whitfield, LS;Mathis, D
TCR：MHC/肽相互作用的动力学特征决定了它们在体外的结果，其中一些重要参数包括参与的分子数量和参与的持续时间。我们探讨了在体内的意义，这些研究结果在转基因小鼠表达TCR的定量和时间控制的方式。正如预期的那样，TCR水平降低导致反应性减弱，但反应阈值明显低于预期-低至正常TCR数量的1/20（th），并且在次优水平下没有表型偏斜的迹象。我们还研究了去除TCR的T淋巴细胞的存活率。与B细胞不同，缺乏抗原受体的T细胞不会突然死亡;相反，群体逐渐衰减，就像以前在缺乏MHC分子的情况下报道的那样。
Kinetic features of TCR:MHC/peptide interactions dictate their outcome in vitro, some important parameters of which include the number of molecules engaged and the duration of engagement. We explored the in vivo significance of these findings in transgenic mice expressing TCRs in a quantitatively and temporally controlled manner. As anticipated, reduced TCR levels resulted in attenuated reactivity, but response thresholds were substantially lower than expected-at as low as 1/20(th), the normal TCR numbers and with no indication of phenotypic skewing at suboptimal levels. We also studied survival of T lymphocytes stripped of their TCRs. Unlike B cells, T cells lacking antigen receptors did not die precipitously; instead, populations decayed gradually, just as previously reported in the absence of MHC molecules.