Change of mRNA amount of myosin heavy chain in masseter muscle after orthognathic surgery of patients with malocclusion
Change of mRNA amount of myosin heavy chain in masseter muscle after orthognathic surgery of patients with malocclusion
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DOI：
10.1016/s1010-5182(06)60023-1
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Harzer, Winfried
中科院分区：
文献类型：
--
作者：
Gedrange, Tomas;Buettner, Cordula;Harzer, Winfried
简介：错牙合畸形的手术矫治是通过截骨后骨的缩短、延长和旋转改变附着于下颌骨的咀嚼肌的力与力矩的比值。在肌肉适应过程中，I型和II型肌纤维的肌球蛋白重链（M.yHC）的mRNA表达可能发生变化。材料和方法：在mRNA水平上研究了10名患者在下颌骨正颌手术后6个月的咬肌适应性。竞争性聚合酶链反应（cPCR）是一种合适的方法来定量MyHC mRNA。对于这种微创方法的应用，35 mg肌肉组织的量是足够的。结果：术后6个月，两组患者中I型和II型纤维的MyHC mRNA均缺失约87%。下颌骨近中位患者的缺失率较高，但与远中位患者差异无统计学意义。结论：患者应充分利用术后间隙进行咀嚼肌训练。这提高了治疗结果的稳定性并防止复发。(c)2006年欧洲颅颌面外科协会。
Introduction: Surgical correction of malocclusion changes the force to moment ratio of masticatory muscles inserting at the mandible caused by shortening, lengthening and rotation of the bone following osteotomy. During muscle adaptation the expression of mRNA for the myosin heavy chain (M.yHC) of type I and type II fibres may be changed. Material and methods: The adaptation of the masseter muscle was investigated at the mRNA level in 10 patients 6 months after orthognathic surgery in the mandible. The competitive polymerase chain reaction (cPCR) is a suitable method for quantification of MyHC mRNA. For application of this minimal invasive method an amount of 35 mg muscle tissue was sufficient. Results: 6 month postoperatively there was a deficiency of about 87% of MyHC mRNA for fibre type I and II in both groups of patients. The deficiency in patients with mesial position of the mandible was higher but not significant different to patients with distall malocclusion. Conclusion: Patients should use the postoperative interval for training their masticatory muscles. This improves the stability of treatment result and prevents relapse. (c) 2006 European Association for Cranio-Maxillofacial Surgery.