Nucleotide sequence of the Acinetobacter calcoaceticus trpGDC gene cluster.
Nucleotide sequence of the Acinetobacter calcoaceticus trpGDC gene cluster.
复制标题
乙酸钙不动杆菌 trpGDC 基因簇的核苷酸序列。
DOI：
10.1093/oxfordjournals.molbev.a040331
复制
发表时间：
1984
影响因子：
10.7
通讯作者：
Nichols,BP
中科院分区：
文献类型：
--
作者：
Kaplan,JB;Goncharoff,P;Seibold,AM;Nichols,BP
A plasmid library of Acinetobacter calcoaceticus HindIII fragments was constructed, and clones that complemented an Escherichia coli pabA mutant were selected. Plasmids containing a 3.9-kb fragment of A. calcoaceticus DNA that also complemented E. coli trpD and trpC-(trpF+) mutants were obtained. We infer that complementation of E. coli pabA mutants was the result of the expression of the amphibolic anthranilate-synthase/p-aminobenzoate-synthase glutamine-amidotransferase gene and that the plasmid insert carried the entire trpGDC gene cluster. In E. coli minicells, the plasmid insert directed the synthesis of polypeptides of 44,000, 33,000, and 20,000 daltons, molecular masses that are consistent with the reported molecular masses of phosphoribosylanthranilate transferase, indoleglycerol-phosphate synthase, and anthranilate-synthase component II, respectively. A 3,105-bp nucleotide sequence was determined. Comparison of the A. calcoaceticus trpGDC sequences with other known trp gene sequences has allowed insight into (1) the evolution of the amphibolic trpG gene, (2) varied strategies for coordinate expression of trp genes, and (3) mechanisms of gene fusions in the trp operon.
