SCHISTOSOMA-MANSONI - MYOGENIC CHARACTERISTICS OF PHORBOL ESTER-INDUCED MUSCLE-CONTRACTION
SCHISTOSOMA-MANSONI - MYOGENIC CHARACTERISTICS OF PHORBOL ESTER-INDUCED MUSCLE-CONTRACTION
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DOI：
10.1006/expr.1994.1032
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发表时间：
1994-05-01
影响因子：
2.1
通讯作者：
PAX, RA
中科院分区：
文献类型：
--
作者：
BLAIR, KL;BENNETT, JL;PAX, RA
应用肌肉生理学和电生理技术研究了佛波酯诱发的曼氏血吸虫肌肉挛缩的肌源性。挛缩依赖于细胞外Ca 2+，被Ca 2+通道阻滞剂阻断，并且似乎与肌肉对Ca 2+的渗透性增加有关，但与Na+或H+无关。在佛波酯引起的挛缩中，肌肉组织没有去极化，但表面电活动减少。阈值处理佛波酯和去极化或吡喹酮产生协同挛缩的寄生虫。挛缩不能解释为改变释放或敏感性假定的神经递质，减少Ca 2+流出，或增加的敏感性的收缩系统的Ca 2+。这些结果支持的假设，蛋白激酶-C的激活与佛波醇酯导致肌肉挛缩，通过增强肌膜Ca 2+通道的活性。(C)1994年出版社出版。
The myogenic nature of phorbol ester-induced muscle contractures of Schistosoma mansoni was investigated using muscle physiology and electrophysiological techniques. The contracture is dependent of extracellular Ca2+, is blocked by Ca2+ channel blockers, and appears to be associated with an increase in the permeability of the muscle to Ca2+ but not to Na+ or H+. The musculature is not depolarized during phorbol ester-induced contracture, but surface electrical activity decreases. Threshold treatments of phorbol ester and depolarization or praziquantel produce synergistic contractures of the parasite. The contracture could not be explained by altered release of or sensitivity to putative neurotransmitters, decreased Ca2+ efflux, or an increase in the sensitivity of the contractile system to Ca2+. These results support the hypothesis that activation of protein kinase-C in the schistosome with phorbol esters leads to muscle contracture by enhancing sarcolemmal Ca2+ channel activity. (C) 1994 Academic Press, Inc.