MONOCLONAL-ANTIBODIES TO A SYNTHETIC FIBRIN-LIKE PEPTIDE BIND TO HUMAN FIBRIN BUT NOT FIBRINOGEN
MONOCLONAL-ANTIBODIES TO A SYNTHETIC FIBRIN-LIKE PEPTIDE BIND TO HUMAN FIBRIN BUT NOT FIBRINOGEN
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DOI：
10.1126/science.6648524
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发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
MATSUEDA, GR
中科院分区：
文献类型：
--
作者：
HUI, KY;HABER, E;MATSUEDA, GR
从β的氨基末端合成的七肽。人纤维蛋白中的链被用作抗原，以产生与纤维蛋白结合的单抗，即使在血浆中发现的浓度的人纤维蛋白原存在的情况下也是如此。正如预期的那样，抗纤蛋白活性被多肽抗原抑制，但不被对照七肽抑制。在鸡的体外循环模型中检测纤维蛋白，静脉注射。给予与放置在体外小室中的人纤维蛋白涂层圆盘结合的单抗。这些发现可能导致更好的方法来识别深静脉和冠状动脉血栓。
A synthetic heptapeptide from the amino terminus of the .beta. chain in human fibrin was used as an antigen to produce monoclonal antibodies that bind to fibrin even in the presence of human fibrinogen at the concentration found in plasma. As expected, the antifibrin activity was inhibited by the peptide antigen but not by a control heptapeptide. In a chicken ex vivo circulatory model for fibrin detection, i.v. administered monoclonal antibodies bound to human fibrin-coated disks placed in an extracorporeal chamber. These findings may lead to better methods for identifying deep vein and coronary artery thrombi.