Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell.
Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell.
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DOI：
10.1155/2016/6924593
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发表时间：
2016
影响因子：
4.3
通讯作者：
Sun ZL
中科院分区：
文献类型：
--
作者：
Li FF;Chen BJ;Li W;Li L;Zha M;Zhou S;Bachem MG;Sun ZL
我们以前分离胰岛星状细胞（ISCs）从健康的Wistar大鼠胰岛。在本研究中，我们从Goto-Kakizaki大鼠的胰岛中分离出“已经被糖尿病环境引发”的ISCs，确定这些细胞的基因谱，并评估这些ISCs对β细胞功能和存活的影响。我们通过数字基因表达检测ISC的基因表达。用ISCs上清液（SN）处理INS-1细胞后，检测INS-1细胞增殖、凋亡和胰岛素分泌。我们观察到ISCs和PSC的表达模式相似，但有1067个差异表达基因。ISC-SN培养的INS-1细胞中胰岛素的产生显著减少。ISC-SN处理后5-乙炔基-2 ′-脱氧尿苷阳性的INS-1细胞减少。碘化丙啶（PI-）阳性INS-1细胞是对照组的2.6倍。Caspase-3活性增加。结论：GK大鼠纤维化胰岛中存在的ISCs可能是一种特殊的PSC，其功能和增殖受损，凋亡增加。
We previously isolated islet stellate cells (ISCs) from healthy Wistar rat islets. In the present study, we isolated “already primed by diabetic environment” ISCs from islets of Goto-Kakizaki rats, determined the gene profile of these cells, and assessed the effects of these ISCs on beta-cell function and survival. We detected gene expression of ISCs by digital gene expression. INS-1 cell proliferation, apoptosis, and insulin production were measured after being treated with ISCs supernatant (SN). We observed the similar expression pattern of ISCs and PSCs, but 1067 differentially expressed genes. Insulin production in INS-1 cells cultured with ISC-SN was significantly reduced. The 5-ethynyl-2′-deoxyuridine-positive INS-1 cells treated with ISC-SN were decreased. Propidium iodide- (PI-) positive INS-1 cells were 2.6-fold higher than those in control groups. Caspase-3 activity was increased. In conclusion, ISCs presented in fibrotic islet of GK rats might be special PSCs, which impaired beta-cell function and proliferation and increased beta-cell apoptosis.