Genome-enabled analysis of the utilization of taurine as sole source of carbon or of nitrogen by Rhodobacter sphaeroides 2.4.1
Genome-enabled analysis of the utilization of taurine as sole source of carbon or of nitrogen by Rhodobacter sphaeroides 2.4.1
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DOI：
10.1099/mic.0.29195-0
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发表时间：
2006-11-01
期刊：
影响因子：
2.8
通讯作者：
Cook, Alasdair M.
中科院分区：
文献类型：
--
作者：
Denger, Karin;Smits, Theo H. M.;Cook, Alasdair M.
Bruggemann埃塔尔提出了一种在球形红细菌2.4.1中降解牛磺酸（2-氨基乙磺酸）的途径。（2004）（Microbiology 150，805-816）基于部分基因组序列，在本研究中，R.发现sphaeroides 2.4.1以牛磺酸作为生长的唯一碳源和能量源呈指数生长。当牛磺酸是琥珀酸盐培养基中唯一的氮源时，牛磺酸迅速降解，大部分有机氮以铵离子的形式排出，然后用于生长。大多数参与异化的酶，牛磺酸脱氢酶（TDH），磺基乙醛乙酰转移酶（XSC）和磷酸乙酰转移酶（Pta），被发现是诱导型的，并通过逆转录PCR获得相应基因（tauXY XSC和pta），以及tauKLM，编码牛磺酸的假定TRAP转运蛋白，和tauZ，编码硫酸盐输出蛋白的转录证据。Novak等人（2004）（Microbiology 150，1881-1891）在R. sphaeroides TAU 3，涉及牛磺酸：丙酮酸氨基转移酶（Tpa）和推定的ABC转运蛋白（NsbABC）。没有证据表明该途径或相应的丙氨酸脱氢酶（Ald）在R. sphaeroides 2.4.1. R. sphaeroides 2.4.1似乎涉及诱导合成的苹果酰辅酶A裂解酶，而不涉及苹果酸合酶（GlcB），其假定基因在这些条件下不转录。
A degradative pathway for taurine (2-aminoethanesulfonate) in Rhodobacter sphaeroides 2.4.1 was proposed by Bruggemann etal. (2004) (Microbiology 150, 805-816) on the basis of a partial genome sequence, In the present study, R. sphaeroides 2.4.1 was found to grow exponentially with taurine as the sole source of carbon and energy for growth. When taurine was the sole source of nitrogen in succinate-salts medium, the taurine was rapidly degraded, and most of the organic nitrogen was excreted as the ammonium ion, which was then utilized for growth. Most of the enzymes involved in dissimilation, taurine dehydrogenase (TDH), sulfoacetalclehyde acetyltransferase (Xsc) and phosphate acetyltransferase (Pta), were found to be inducible, and evidence for transcription of the corresponding genes (tauXY xsc and pta), as well as of tauKLM, encoding the postulated TRAP transporter for taurine, and of tauZ, encoding the sulfate exporter, was obtained by reverse-transcription PCR. An additional branch of the pathway, observed by Novak et al. (2004) (Microbiology 150, 1881-1891) in R. sphaeroides TAU3, involves taurine: pyruvate aminotransferase (Tpa) and a presumptive ABC transporter (NsbABC). No evidence for a significant role of this pathway, or of the corresponding alanine dehydrogenase (Ald), was obtained for R. sphaeroides 2.4.1. The anaplerotic pathway needed under these conditions in R. sphaeroides 2.4.1 seems to involve malyl-CoA lyase, which was synthesized inducibly, and not malate synthase (GlcB), whose presumed gene was not transcribed under these conditions.