Improvement of drug elution in thin mineralized collagen coatings with PLGA-PEG-PLGA micelles
Improvement of drug elution in thin mineralized collagen coatings with PLGA-PEG-PLGA micelles
复制标题
使用 PLGA-PEG-PLGA 胶束改善薄矿化胶原蛋白涂层中的药物洗脱。
DOI：
10.1002/jbm.a.34625
复制
发表时间：
2013-11-01
影响因子：
4.9
通讯作者：
Du, Piyi
中科院分区：
文献类型：
--
作者：
Ling, Ting;Yu, Mengfei;Du, Piyi
金属植入物上的矿化胶原（MC）涂层由于其高生物学反应而显示出作为骨科材料的巨大潜力。然而，它们的药物递送能力仍然不令人满意，因为可能发生严重的突释并且难以实现长期释放。为了提高药物洗脱能力，我们将载药PLGA-PEG-PLGA（PPP）胶束加入到薄涂层中。体外释药曲线显示，改性后的包衣片在初始8 h内的突释量较MC包衣片从81%下降到58%，同时释放持续时间从3 d延长到1周。此外，盐酸万古霉素（VH，模型药物）的释放动力学可以通过改变PPP胶束的大小和浓度来调节。有趣的是，根据抗菌测试，胶束固定引起的VH的较少初始释放并不影响植入早期阶段的抗菌活性。细胞相容性实验表明，负载VH的PPP胶束对涂层的生物活性没有负面影响，与裸Ti基底相比，涂层大大提高了细胞活性。因此，具有PPP胶束的MC涂层可能是用于骨修复的有效植入途径。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：3256-3265，2013.
A mineralized collagen (MC) coating on metallic implants has shown great potential as orthopedic material due to high biological responses. However, their drug delivery capacity remains unsatisfactory since a serious burst release may occur and long-term release is hard to be achieved. Aiming to improve the drug-eluting capability, we incorporated drug-loaded PLGA-PEG-PLGA (PPP) micelles into the thin coating. The in vitro release profiles showed that the burst release in the initial 8 h of the modified coating decreased from 81% to 58% compared to MC coating alone; meanwhile, the release duration was prolonged from 3 days to 1 week. Additionally, the release kinetics of vancomycin hydrochloride (VH, the model drug) could be adjusted by changing the size and concentration of PPP micelles. Interestingly, less initial release of VH caused by micelle immobilization did not affect the antibacterial activity in the early stage of implantation according to the antimicrobial test. The cytocompatibility assay demonstrated that the VH-loaded PPP micelles did not have negative effect on the bioactivity of coating which greatly enhanced cell activity compared to bare Ti substrates. Thus, the MC coatings with PPP micelles could be an effective implant route for bone repair. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3256-3265, 2013.