3-D reconstruction of the vestibular endorgans in pediatric temporal bones
3-D reconstruction of the vestibular endorgans in pediatric temporal bones
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DOI：
10.1016/s0378-5955(03)00255-7
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发表时间：
2003-11-01
期刊：
影响因子：
2.8
通讯作者：
Bachor, E
中科院分区：
文献类型：
--
作者：
Rother, T;Schröck-Pauli, C;Bachor, E
我们调查了前庭终器在三个孩子使用3-D重建组织切片。将无外周前庭病变的新生儿的右颞骨用作参考模型，并研究了来自Goldenhar综合征儿童和具有已知外周前庭病变的Pierre Robin序列儿童的颞骨。所有5个颞骨均通过火棉胶技术制备，并在20 μ m处切片。用载玻片扫描仪对每个可用切片进行数字化。成像数据按照解剖学正确分层并在3D软件中渲染。用这种技术重建和测量所有前庭终器。距离的标准差范围为0.5 - 1.2%，角度范围为0.1 - 2.9度。两个黄斑在纵轴和横轴上均呈35 °左右的弯曲。半规管之间的夹角在97度和110度之间变化。病理模型显示半规管的扭曲配置，并在大多数测量的距离和角度与参考模型有很大不同。所提出的方法是能够产生的三维模型的前庭系统的组织切片具有可接受的精度，而无需事先插入的参考标记。档案火棉胶部分是广泛可用的，将是一个重要的资源，在了解详细的三维几何形状的前庭系统尚未完成。(C)2003 Elsevier B. V.保留所有权利。
We investigated the vestibular endorgans in three children using 3-D reconstructions from histological sections. The right temporal bone of a newborn child without peripheral vestibular pathology was used as reference model and the temporal bones from a child with Goldenhar syndrome and a child with Pierre Robin sequence with known peripheral vestibular pathology were studied. All five temporal bones were prepared by the celloidin technique and sectioned at 20 mum. Each available section was digitized with a slide scanner. The imaging data were layered anatomically correctly and rendered in a 3-D software. With this technique all vestibular endorgans were reconstructed and measured. The standard deviations in distances ranged between 0.5 and 1.2% and in angles between 0.1 and 2.9degrees. Both maculae were curved in the longitudinal and transverse axes which described a curve of approximately 35degrees. The angles between the semicircular ducts varied between 97 and 110degrees. The pathological models demonstrated a distorted configuration of the semicircular canals and differed substantially from the reference model in most of the measured distances and angles. The method presented is capable of generating 3-D models of the vestibular system from histological sections with an acceptable precision without previously inserted reference marks. Archival celloidin sections are widely available and will be an important resource in understanding the detailed 3-D geometry of the vestibular system which has not yet been accomplished. (C) 2003 Elsevier B.V. All rights reserved.