Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis
Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis
复制标题
肺部恶化抗生素治疗对囊性纤维化细菌多样性的影响
DOI：
10.1016/j.jcf.2012.05.008
复制
发表时间：
2013-01-01
影响因子：
5.2
通讯作者：
Carroll, M. P.
中科院分区：
文献类型：
--
作者：
Daniels, T. W. V.;Rogers, G. B.;Carroll, M. P.
背景：除了那些被认为具有临床重要性的细菌外，CF气道中还存在各种各样的细菌。在此，我们研究了抗生素对其他非假单胞菌的相对影响，这些抗生素主要用于急性加重期的铜绿假单胞菌。方法：在抗生素治疗前21、14和7天，治疗第3天，治疗最后一天，结果：总体而言，与其他细菌种类相比，抗生素治疗期间铜绿假单胞菌的相对丰度增加;抗生素治疗前的平均丰度为51.0 ± 36.0%，抗生素治疗期间为71.3 ± 30.4%（ANOVA：F-1，F-54=5.16; P
Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species.Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7 days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14 days afterwards, by T-RFLP profiling.Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0 +/- 36.0% increasing to 71.3 +/- 30.4% during antibiotic (ANOVA: F-1,F-54=5.16; P