Comparing polyvalent bacteriophage and bacteriophage cocktails for controlling antibiotic-resistant bacteria in soil-plant system
Comparing polyvalent bacteriophage and bacteriophage cocktails for controlling antibiotic-resistant bacteria in soil-plant system
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比较多价噬菌体和噬菌体混合物用于控制土壤-植物系统中的抗生素抗性细菌
DOI：
10.1016/j.scitotenv.2018.11.457
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发表时间：
2019
影响因子：
9.8
通讯作者：
Hu Feng
中科院分区：
文献类型：
--
作者：
Zhao Yuanchao;Ye Mao;Zhang Xinting;Sun Mingming;Zhang Zhongyun;Chao Huizhen;Huang Dan;Wan Jinzhong;Zhang Shengtian;Jiang Xin;Sun Dawei;Yuan Yilin;Hu Feng
抗生素耐药病原菌(ARPB)在土壤-植物系统中的残留对公众健康和环境安全造成了严重威胁。噬菌体疗法能够靶向裂解宿主细菌，被认为是控制环境中ARPB污染的一种有前途的方法。本研究通过微宇宙实验研究了不同噬菌体处理对土壤-胡萝卜系统中四环素抗性大肠埃希菌和氯霉素抗性铜绿假单胞菌PAO1消散的影响。孵育70 天后，4个噬菌体处理均显著降低了土壤-胡萝卜系统中病原菌及其相应的抗生素抗性基因( 和 )的丰度(pΦ<； + Φ) >； 多价噬菌体(ΦYSZ3宽寄主噬菌体)处理 >； 宿主特异性噬菌体(ΦYSZ2和ΦYSZ1)处理 >； 为对照。此外，多价噬菌体处理还对系统中细菌群落的多样性和稳定性产生了积极的影响，这表明这是一种环境友好的技术，在土壤-植物系统中ARPB/ARGs的生物控制中具有广泛的应用前景。
Antibiotic resistant pathogenic bacteria (ARPB) residual in soil-plant system has caused serious threat against public health and environmental safety. Being capable of targeted lysing host bacteria, phage therapy has been proposed as promising method to control the ARPB contamination in environments. In this study, microcosm trials were performed to investigate the impact of various phage treatments on the dissipation of tetracycline resistantEscherichia coliK-12 and chloramphenicol resistantPseudomonasaeruginosaPAO1 in soil-carrot system. After 70 days of incubation, all the four phage treatments significantly decreased the abundance of the pathogenic bacteria and the corresponding antibiotic resistance genes (tetW and cmlA) in the soil-carrot system (p < 0.05), following the order of the cocktail phage treatment (phages ΦYSZ1 + ΦYSZ2) > the polyvalent phage (ΦYSZ3 phage with broad host range) treatment > host-specific phage (ΦYSZ2 and ΦYSZ1) treatments > the control. In addition, the polyvalent phage treatment also exerted positive impact on the diversity and stability of the bacterial community in the system, suggesting that this is an environmentally friendly technique with broad applications in the biocontrol of ARPB/ARGs in soil-plant system.