Window-period human immunodeficiency virus transmission to two recipients by an adolescent blood donor
Window-period human immunodeficiency virus transmission to two recipients by an adolescent blood donor
复制标题
DOI：
10.1111/j.1537-2995.2004.03364.x
复制
发表时间：
2004-06-01
期刊：
影响因子：
2.9
通讯作者：
McKenna, M
中科院分区：
文献类型：
--
作者：
Phelps, R;Robbins, K;McKenna, M
背景：联合NAT和献血者筛查将献血者人群中HIV的诊断窗口期缩短至约10至15天。本报告描述了两例输血获得性艾滋病毒感染病例和从供体向受者传播的核实情况，并试图确定这名18岁的供体是如何感染的。研究设计和方法：在重复供体HIV检测结果呈阳性后，确定两名供体先前供体的接受者并进行检测。对供体和受者进行访谈，并采集血样进行HIV DNA测序和系统发育分析。结果：2例患者HIV检测结果均为阳性。系统发育分析显示，病毒之间具有很高的遗传相似性（bootstrap为100%），与从供体到受体的传播一致。在最可能发生感染的关键时期，与献血者有过性接触的五名男子中有四名被找到并进行了检测；结果为HIV阴性。结论：献血者集合NAT并没有消除血清转化过程中HIV检测的窗口期。
BACKGROUND: Pooled NAT and donor screening have reduced the diagnostic window period for HIV in the blood donor population to approximately 10 to 15 days. This report describes two cases of transfusion-acquired HIV infection and verification of transmission from the donor to the recipients, and attempts to identify how the 18-year-old donor acquired her infection.STUDY DESIGN AND METHODS: After a repeat donor had a positive HIV test result, two recipients of the donor's previous donation were identified and tested. The donor and recipients were interviewed and blood samples were obtained for HIV DNA sequencing and phylogenetic analysis.RESULTS: The two recipients had positive HIV test results. Phylogenetic analysis showed a high genetic similarity among the viruses (bootstrap 100%), consistent with transmission from the donor to the recipients. Four of five men with whom the donor had sexual contact during the critical time period when infection most likely occurred were located and tested; results were negative for HIV.CONCLUSIONS: Pooled NAT of blood donations has not eliminated the window period for HIV identification during seroconversion.