Variability in plasma prothrombin concentration: implications for use in epidemiology.
Variability in plasma prothrombin concentration: implications for use in epidemiology.
复制标题
血浆凝血酶原浓度的变异性：对流行病学应用的影响。
DOI：
10.1097/00001721-199809000-00010
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Tracy,RP
中科院分区：
文献类型：
--
作者：
Smiles,AM;Macy,EM;Sakkinen,PA;Bovill,EG;Mann,KG;Tracy,RP
Recent evidence suggests a major role for prothrombin as a risk factor for thrombosis. However, estimating prothrombin levels from a deficient plasma-based clotting assay (factor lie) is expensive and technically difficult in the setting of population-based research. We report the development of an enzyme-linked immunosorbent assay (ELISA) for prothrombin using purified antigen and polyclonal anti-prethrombin-1 IgG. Three different quality control plasmas had coefficients of variation (CV) of 6.5%, 4.9%, and 4.8%. Analytical recovery averaged 103.8%. Results from the ELISA correlated well with factor lie results (r= 0.75). The 5th-95th percentile range for healthy men (n= 10) and women (n= 16) was 97.7 μg/mI to 161.8 μg/ml. The assay exhibited no significant cross-reactivity with other vitamin-K-dependent proteins. Prothrombin showed no diurnal variation. In a study of biovariability the analytical variability, CV A, was 3.1%; the within-subject variability, CV I, was 7.3%; the between-subject variability, CV G, was 14.5%. The critical difference for sequential values (ie the smallest percentage change unlikely to be due to CV A or CV I) significant at P= 0.05 was 21.9%. The index of individuality, CV I/CV G, was 0.50. On the basis of the overall biovariability data, primarily the index of individuality, prothrombin as measured in our ELISA is well suited for applications in population-based research.
