Cis-acting sequences responsive to the rev gene product of the human immunodeficiency virus.
Cis-acting sequences responsive to the rev gene product of the human immunodeficiency virus.
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发表时间：
1988
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通讯作者：
Andrew Dayton;Ernest F. Terwilliger;J. Potz;Mark M. Kowalski;Joseph G. Sodroski;W. Haseltine
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作者：
Andrew Dayton;Ernest F. Terwilliger;J. Potz;Mark M. Kowalski;Joseph G. Sodroski;W. Haseltine
人类免疫缺陷病毒1型（HIV-1）结构蛋白的高水平表达需要两种调节基因的存在，即反式激活因子（达特）和病毒体蛋白表达调节因子（以前称为art或trs）。这里描述的实验表明，病毒体蛋白的表达依赖于位于包膜基因中的一个小区域，称为顺式作用抗阻遏序列（CAR）。包膜序列的CAR区域对于Rev依赖性衣壳蛋白表达是必要的和足够的。实验还表明，rev或CAR的缺陷导致基因组和包膜mRNA的积累急剧减少，以及更广泛剪接的病毒mRNA种类的过度产生。
Expression of high levels of the structural proteins of the human immunodeficiency virus type 1 (HIV-1) requires the presence of two regulatory genes, the trans-activator (tat), and the regulator of virion protein expression (rev. previously called art or trs). The experiments described here show that expression of virion proteins is dependent upon a small region located in the envelope gene called the cis-acting antirepression sequence (CAR). The CAR region of the envelope sequence is both necessary and sufficient for rev-dependent capsid protein expression. The experiments also show that a defect in either rev or CAR results in a dramatic decrease in the accumulation of the genomic and envelope mRNAs and an overproduction of more extensively spliced viral mRNA species.