Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
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DOI：
10.1007/978-1-4939-6887-9_5
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Esposito, Dominic
中科院分区：
文献类型：
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作者：
Taylor, Troy;Denson, John-Paul;Esposito, Dominic
任何蛋白质表达实验的主要目标是将每个细胞可溶性蛋白质的最大产量与最高可能的细胞密度相结合，以最有效地获得高产量的蛋白质。在这些实验中可以优化大量的参数，但是对每细胞生产率和细胞密度具有强烈影响的最有趣的参数之一是与表达诱导过程偶联的细胞生长培养基。利用特殊培养基和多种诱导条件，有可能显著提高E.杆菌
The major goal of any protein expression experiment is to combine the maximum production per cell of soluble protein with the highest possible cell density to most efficiently obtain high yields of protein. A large number of parameters can be optimized in these experiments, but one of the most interesting parameters that have a strong effect on both per cell productivity and cell density is the cellular growth media coupled to the expression induction process. Using specialized media and testing multiple induction conditions, it is possible to significantly enhance the production of heterologous proteins from E. coli.