Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution and yoctomole mass detection limits in a 32-channel system.
Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution and yoctomole mass detection limits in a 32-channel system.
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DOI：
10.1007/s00216-010-3595-x
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发表时间：
2010-08
影响因子：
4.3
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Dada, Oluwatosin O.;Ramsay, Lauren M.;Dickerson, Jane A.;Cermak, Nathan;Jiang, Rong;Zhu, Cuiru;Dovichi, Norman J.
关键词：
我们报告了一个多重毛细管电泳系统采用了32毛细管阵列与微机械鞘流池作为检测室。用473 nm激光束同时激发样品流，并将荧光发射在具有一对双重消色差透镜的CCD照相机上成像。该仪器对荧光素的区带电泳质量检测限为380 ± 120 μ mol。荧光标准品的毛细管等电聚焦产生平均宽度为0.0029 ± 0.0008 pH的峰。毛细管涂层稳定性限制了分析的重现性。
We report a multiplexed capillary electrophoresis system employing an array of 32 capillaries with a micromachined sheath flow cuvette as the detection chamber. The sample streams were simultaneously excited with a 473 nm laser beam, and the fluorescence emission was imaged on a CCD camera with a pair of doublet achromat lens. The instrument produced mass detection limits of 380 ± 120 yoctomoles for fluorescein in zone electrophoresis. Capillary isoelectric focusing of fluorescent standards produced peaks with an average width of 0.0029 ± 0.0008 pH. Capillary coating stability limits the reproducibility of the analysis.
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影响因子：
7.4
作者：
Zhang, JZ;Yang, MJ;Dovichi, NJ
通讯作者：
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影响因子：
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Dovichi, Norman J.
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