ROLES OF H-1 DOMAINS IN DETERMINING HIGHER-ORDER CHROMATIN STRUCTURE AND H-1 LOCATION
ROLES OF H-1 DOMAINS IN DETERMINING HIGHER-ORDER CHROMATIN STRUCTURE AND H-1 LOCATION
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DOI：
10.1016/0022-2836(86)90337-2
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发表时间：
1986-02-20
影响因子：
5.6
通讯作者：
CRANEROBINSON, C
中科院分区：
文献类型：
--
作者：
ALLAN, J;MITCHELL, T;CRANEROBINSON, C
源自小牛胸腺 H1 和大鼠肝脏 H1 的肽仅包含完整分子的球状和 COOH 末端结构域，因此缺乏 NH2 末端结构域，通过重建已证明在诱导高阶染色质结构方面与完整 H1 分子一样有效。由于 H1 的球状结构域单独不能诱导染色质折叠，我们的结果表明该功能主要由分子的 COOH 末端结构域控制。令人惊讶的是，这些肽相对于核小体没有正确定位。当用微球菌核酸酶消化时，它们未能赋予重组体保护染色体长度的DNA片段的能力，这证明了这一点。 H1 分子（球状结构域）相对于核小体的精确位置显示受到 H1 和核心组蛋白的“尾部”结构域的影响。
Peptides derived from calf thymus H1 and rat liver H1, comprising only the globular and COOH-terminal domains of the intact molecule and therefore lacking NH2-terminal domains, have been shown by reconstruction to be as effective as the complete H1 molecule in inducing higher-order chromatin structure. As the globular domain of H1 alone cannot induce chromatin folding, our results demonstrate that this functioon is primarily controlled by the COOH-terminal domain of the molecule. Surprisingly, these peptides do not locate correctly with respect to the nucleosome. This is demonstrated by their failure to confer upon reconstitutes the ability to protect DNA fragments of chromatosome length when digested with micrococcal nuclease. The precise placement of the H1 molecule (globular domain) with respect to the nucleosome is shown to be influenced by the "tail" domains of both H1 and the core histones.